ACP5 Human

Acid Phosphatase-5 Human Recombinant
Cat. No.
BT26476
Source
Sf9, Baculovirus cells.
Synonyms

Acid Phosphatase 5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, Human Purple Acid Phosphatase, EC 3.1.3.2, TrATPase, Tartrate-Resistant Acid Phosphatase Type 5, Tartrate-Resistant Acid Phosphatase 5a, Tartrate-Resistant Acid Phosphatase 5b, Tartrate-Resistant Acid Phosphatase, Type 5 Acid Phosphatase, TRACP5a, TRACP5b, TR-AP, HPAP, TRAP, ACP5.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACP5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 304 amino acids (22-325 a.a.) and having a molecular mass of 34.3kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).
ACP5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Tartrate-resistant acid phosphatase type 5 (ACP5) plays a crucial role in dephosphorylating osteopontin and bone sialoprotein. This iron-containing glycoprotein catalyzes the conversion of orthophosphoric monoester into alcohol and orthophosphate. Increased ACP5 expression is observed in certain disease states, including Gaucher disease, Hodgkin disease, hairy cell leukemia, B-cell leukemia, and T-cell leukemia. As the most basic acid phosphatase, ACP5 is uniquely uninhibited by L(+)-tartrate.

Description
Recombinant human ACP5, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 304 amino acids (22-325 a.a.) and has a molecular weight of 34.3kDa. Under reducing conditions on SDS-PAGE, it migrates between 28-40kDa.
The purification of ACP5 is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation

The ACP5 protein solution is provided at a concentration of 0.5mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. 
To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. 
Repeated freezing and thawing of the product should be avoided.
Purity

The purity of this product is greater than 90.0% as assessed by SDS-PAGE.

Biological Activity

The specific activity of this product exceeds 10,000 units/mg. Specific activity is defined as the amount of enzyme required to hydrolyze 1.0 nanomole of p-nitrophenyl phosphate (pNPP) per minute at a pH of 5.0 and a temperature of 37°C.

Synonyms

Acid Phosphatase 5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, Human Purple Acid Phosphatase, EC 3.1.3.2, TrATPase, Tartrate-Resistant Acid Phosphatase Type 5, Tartrate-Resistant Acid Phosphatase 5a, Tartrate-Resistant Acid Phosphatase 5b, Tartrate-Resistant Acid Phosphatase, Type 5 Acid Phosphatase, TRACP5a, TRACP5b, TR-AP, HPAP, TRAP, ACP5.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP.

Product Science Overview

Structure and Function

ACP5 is an iron-containing glycoprotein that is unique among acid phosphatases due to its resistance to inhibition by L(+)-tartrate . The enzyme’s structure includes a signal peptide (amino acids 1 to 21) and a mature chain (amino acids 22 to 325) . It plays a crucial role in the dephosphorylation of osteopontin and bone sialoprotein, which are important for bone metabolism .

Expression and Pathological States

The expression of ACP5 is notably increased in certain pathological conditions such as Gaucher disease, Hodgkin disease, and various leukemias (hairy cell, B-cell, and T-cell) . This makes it a valuable biomarker for these diseases.

Recombinant Production

Recombinant human ACP5 is produced using a mouse myeloma cell line (NS0-derived) and is typically tagged with a C-terminal 6-His tag for purification purposes . The recombinant protein is highly purified, with a purity greater than 95% as determined by SDS-PAGE . It is used in various research applications, including enzyme activity assays and studies on bone metabolism.

Enzyme Activity

The activity of recombinant ACP5 is measured by its ability to cleave the substrate p-Nitrophenyl phosphate (pNPP). The specific activity is reported to be greater than 65,000 pmol/min/µg under the described conditions . This high level of activity makes it a useful tool for biochemical studies.

Storage and Stability

Recombinant ACP5 is supplied as a filtered solution in Tris and NaCl and should be stored at 2 to 8°C. It is stable for up to three months from the date of receipt when stored under these conditions .

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ACP5 Human, His
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Cat. No.
BT26558
Source
Sf9, Baculovirus cells.
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