ACP5 Human, His

Acid Phosphatase-5 Human Recombinant, His Tag
Cat. No.
BT26558
Source
Sf9, Baculovirus cells.
Synonyms
Acid Phosphatase 5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, EC 3.1.3.2, TrATPase, SPENCDI, Tartrate-Resistant Acid Phosphatase Type 5, Tartrate-Resistant Acid Phosphatase, Type 5 Acid Phosphatase, TR-AP, TRAP, ACP5.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACP5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 310 amino acids (22-325 a.a) and having a molecular mass of 35.1kDa.
ACP5 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Acid Phosphatase-5, also known as ACP5, is a member of the Purple acid phosphatase family. This enzyme is involved in the dephosphorylation of osteopontin and bone sialoprotein. ACP5 expression is elevated in certain pathological conditions, including Gaucher disease, Hodgkin disease, hairy cell leukemia, B-cell leukemia, and T-cell leukemia.
Description
Recombinant human ACP5, expressed in Sf9 insect cells using a baculovirus system, is a single glycosylated polypeptide chain. It consists of 310 amino acids (residues 22-325), resulting in a molecular weight of 35.1 kDa. The protein includes a 6-amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
ACP5 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 95% using SDS-PAGE analysis.
Biological Activity
The specific activity is determined to be greater than 5,000 units/mg. One unit is defined as the amount of enzyme required to hydrolyze 1.0 nanomole of p-nitrophenyl phosphate (pNPP) per minute at a pH of 5.0 and a temperature of 37°C.
Synonyms
Acid Phosphatase 5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, EC 3.1.3.2, TrATPase, SPENCDI, Tartrate-Resistant Acid Phosphatase Type 5, Tartrate-Resistant Acid Phosphatase, Type 5 Acid Phosphatase, TR-AP, TRAP, ACP5.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARPHHHHHH.

Product Science Overview

Structure and Function

ACP5 is an iron-containing glycoprotein and is unique among acid phosphatases because it is not inhibited by L(+)-tartrate . The enzyme plays a crucial role in the dephosphorylation of osteopontin and bone sialoprotein, which are important for bone metabolism . It is also involved in various physiological and pathological processes, including bone resorption, immune response, and the development of certain diseases such as Gaucher and Hodgkin diseases, as well as various leukemias .

Recombinant Production

The recombinant form of ACP5, tagged with a polyhistidine (His) tag, is produced using DNA sequences encoding the human enzyme. This recombinant protein is typically expressed in host cells such as HEK293 cells, which are human embryonic kidney cells . The His tag facilitates the purification of the protein through affinity chromatography, ensuring high purity and activity .

Applications

Recombinant ACP5 is widely used in biochemical research and clinical diagnostics. It serves as a biomarker for bone-related diseases and certain types of cancer. Additionally, it is used in studies related to bone metabolism and immune regulation .

Storage and Stability

Recombinant ACP5 is usually provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C to maintain its stability and activity. It is recommended to avoid repeated freeze-thaw cycles to preserve the protein’s integrity .

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ACP5 Human
Acid Phosphatase-5 Human Recombinant
Cat. No.
BT26476
Source
Sf9, Baculovirus cells.
THE BIOTEK
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