HSPA5 (19-654) Human
Description
HSPA5 (19-654) is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Heat Shock 70kDa Protein 5, also known as HSPA5, is a member of the heat shock protein 70 (HSP70) family. This protein plays a crucial role in the cellular response to stress and is involved in the folding and assembly of proteins within the endoplasmic reticulum (ER). The recombinant form of this protein, specifically the 19-654 amino acid sequence, is often used in research to study its functions and interactions.
The heat shock proteins were first discovered in the 1960s by Ferruccio Ritossa, who observed that heat stress induced the production of specific proteins in Drosophila (fruit flies) . HSPA5, in particular, is localized to the lumen of the ER, where it operates as a chaperone. It assists in the proper folding of nascent proteins and the assembly of multi-protein complexes, ensuring that proteins achieve their correct conformation and functionality .
HSPA5 is a master regulator of ER homeostasis. It is strongly upregulated in response to various stress conditions, including heat stress and exposure to toxic chemicals such as heavy metals (e.g., arsenic, cadmium, copper, mercury) . By preventing the aggregation of misfolded proteins and facilitating their degradation, HSPA5 helps maintain cellular health and function under stress conditions.
The recombinant form of HSPA5, encompassing amino acids 19 to 654, is produced using recombinant DNA technology. This involves inserting the gene encoding HSPA5 into an expression vector, which is then introduced into a host cell (such as bacteria or yeast) to produce the protein. The recombinant protein is purified and used in various research applications, including studies on protein folding, stress response, and ER function .
Recombinant HSPA5 is widely used in biochemical and cell biology research. It serves as a valuable tool for studying the mechanisms of protein folding and the cellular response to stress. Additionally, it is used in drug discovery and development, particularly in the context of diseases associated with protein misfolding and ER stress, such as neurodegenerative disorders and cancer .
