HSPA5 Mouse
78 kDa glucose-regulated protein, GRP-78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, Bip, Hspa5, Grp78, HSPA5, Hsce70.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
HSPA5 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 659 amino acids (20-655 a.a) and having a molecular mass of 72.9kDa. HSPA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
78 kDa glucose-regulated protein, GRP-78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, Bip, Hspa5, Grp78, HSPA5, Hsce70.
MGSSHHHHHH SSGLVPRGSH MGSEEEDKKE DVGTVVGIDL GTTYSCVGVF KNGRVEIIAN DQGNRITPSY VAFTPEGERL IGDAAKNQLT SNPENTVFDA KRLIGRTWND PSVQQDIKFL PFKVVEKKTK PYIQVDIGGG QTKTFAPEEI SAMVLTKMKE TAEAYLGKKV THAVVTVPAY FNDAQRQATK DAGTIAGLNV MRIINEPTAA AIAYGLDKRE GEKNILVFDL GGGTFDVSLL TIDNGVFEVV ATNGDTHLGG EDFDQRVMEH FIKLYKKKTG KDVRKDNRAV QKLRREVEKA KRALSSQHQA RIEIESFFEG EDFSETLTRA KFEELNMDLF RSTMKPVQKV LEDSDLKKSD IDEIVLVGGS TRIPKIQQLV KEFFNGKEPS RGINPDEAVA YGAAVQAGVL SGDQDTGDLV LLDVCPLTLG IETVGGVMTK LIPRNTVVPT KKSQIFSTAS DNQPTVTIKV YEGERPLTKD NHLLGTFDLT GIPPAPRGVP QIEVTFEIDV NGILRVTAED KGTGNKNKIT ITNDQNRLTP EEIERMVNDA EKFAEEDKKL KERIDTRNEL ESYAYSLKNQ IGDKEKLGGK LSSEDKETME KAVEEKIEWL ESHQDADIED FKAKKKELEE IVQPIISKLY GSGGPPPTGE EDTSEKDEL
Product Science Overview
HSPA5 is a multifunctional protein that participates in several cellular processes:
- Protein Folding and Assembly: HSPA5 assists in the folding of newly synthesized proteins and the assembly of multimeric protein complexes within the ER. It interacts with other chaperones and co-chaperones to ensure proteins achieve their correct conformation .
- Calcium Homeostasis: HSPA5 helps maintain calcium balance within the ER, which is essential for various cellular functions .
- ER Stress Response: During conditions of ER stress, such as heat shock or the presence of misfolded proteins, HSPA5 is upregulated to help manage the increased load of unfolded proteins. It binds to misfolded proteins, preventing their aggregation and facilitating their degradation .
- Signal Transduction: HSPA5 has been implicated in signal transduction pathways by acting as a receptor or co-receptor at the plasma membrane .
HSPA5 is essential for various developmental processes and has been implicated in several diseases:
- Embryonic Development: HSPA5 is crucial for the formation of the pronephros, an early kidney structure in vertebrates. It mediates retinoic acid signaling, which is necessary for the development of this organ .
- Cancer: HSPA5 is often overexpressed in cancer cells, where it helps them cope with the increased protein synthesis and folding demands. It also plays a role in tumor progression and resistance to therapy .
- Neurodegenerative Diseases: Given its role in protein folding, HSPA5 is involved in the pathogenesis of neurodegenerative diseases characterized by protein misfolding and aggregation, such as Alzheimer’s disease .
Recombinant HSPA5 from mice is commonly used in research to study its function and role in various biological processes. The recombinant protein is produced using genetic engineering techniques, where the HSPA5 gene is cloned and expressed in a suitable host system, such as bacteria or mammalian cells. This allows researchers to obtain large quantities of the protein for experimental purposes.
Recombinant HSPA5 is used in various research applications, including:
- Protein-Protein Interaction Studies: Researchers use recombinant HSPA5 to study its interactions with other proteins, which helps elucidate its role in protein folding and ER stress response .
- Drug Screening: HSPA5 is a target for drug development, particularly in cancer therapy. Recombinant HSPA5 is used to screen potential inhibitors that could disrupt its function and sensitize cancer cells to treatment .
- Functional Assays: Recombinant HSPA5 is used in functional assays to study its role in calcium homeostasis, signal transduction, and other cellular processes .
