HSPA5 Antibody
Description
Product Specs
Product Science Overview
Heat Shock 70kDa Protein 5 (HSP70) is a member of the heat shock protein family, which plays a crucial role in protein folding, protection against stress, and cellular homeostasis. The HSP70 family is highly conserved across species, including humans and mice. The Mouse Anti-Human HSP70 antibody is a monoclonal antibody used in various research applications to detect and study the HSP70 protein in human tissues and cells.
HSP70 proteins are characterized by their molecular weight of approximately 70 kDa. They consist of two main domains: the N-terminal ATPase domain and the C-terminal substrate-binding domain. The ATPase domain is responsible for binding and hydrolyzing ATP, which provides the energy required for the protein’s chaperone activity. The substrate-binding domain interacts with unfolded or misfolded proteins, preventing their aggregation and assisting in their proper folding.
HSP70 proteins are involved in several cellular processes, including:
- Protein folding and assembly: HSP70 assists in the proper folding of newly synthesized proteins and the refolding of denatured proteins.
- Protein transport: HSP70 facilitates the translocation of proteins across cellular membranes.
- Protection against stress: HSP70 is upregulated in response to various stress conditions, such as heat shock, oxidative stress, and inflammation, to protect cells from damage.
- Regulation of apoptosis: HSP70 can inhibit apoptosis by interacting with key components of the apoptotic pathway.
The Mouse Anti-Human HSP70 antibody is typically produced using hybridoma technology. This involves the following steps:
- Immunization: Mice are immunized with human HSP70 protein or peptides to elicit an immune response.
- Cell Fusion: Spleen cells from the immunized mice, which produce antibodies, are fused with myeloma cells to create hybridoma cells. These hybridoma cells can proliferate indefinitely and produce large quantities of the desired antibody.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies that specifically recognize human HSP70. Positive clones are selected and expanded.
- Purification: The antibodies are purified from the hybridoma cell culture supernatant using techniques such as protein A or protein G affinity chromatography.
The Mouse Anti-Human HSP70 antibody is widely used in various research applications, including:
- Western Blotting: To detect and quantify HSP70 protein levels in cell and tissue lysates.
- Immunohistochemistry: To visualize the localization and expression of HSP70 in tissue sections.
- Flow Cytometry: To analyze HSP70 expression in individual cells.
- Immunoprecipitation: To isolate HSP70 protein complexes from cell lysates for further analysis.
