HPGD Human
Hydroxyprostaglandin Dehydrogenase 15-(NAD) Human Recombinant
Cat. No.
BT9756
Source
Escherichia Coli.
Synonyms
GDH1, SDR36C1, 15-PGDH, PGDH, Prostaglandin dehydrogenase 1, hydroxyprostaglandin dehydrogenase 15-(NAD)
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HPGD Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 286 amino acids (1-266a.a.) and having a molecular mass of 31.1 kDa. HPGD is fused to a 20 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
HPGD, or 15-PGDH, is the primary enzyme responsible for prostaglandin degradation. It significantly reduces the biological activity of prostaglandins by catalyzing the oxidation of their 15-hydroxyl group into a keto group. This enzyme plays a crucial role in various physiological and cellular processes, including inflammation.
Description
This recombinant HPGD protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 286 amino acids (residues 1-266), with a molecular weight of 31.1 kDa. The protein includes a 20 amino acid His-Tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The HPGD protein is supplied in a solution at a concentration of 0.5 mg/ml. The formulation buffer consists of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of this protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
GDH1, SDR36C1, 15-PGDH, PGDH, Prostaglandin dehydrogenase 1, hydroxyprostaglandin dehydrogenase 15-(NAD)
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG IHFQDYDTTP FQAKTQ.
Product Science Overview
Function and Mechanism
15-PGDH is a NAD±linked dehydrogenase that catalyzes the oxidation of the hydroxyl group at the 15th position of prostaglandins to a ketone, leading to a loss of their biological activity . This reaction is essential for the regulation of prostaglandin levels in various tissues, thereby modulating inflammation, pain, and other physiological processes.
Structure
Applications
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