HPGD Mouse
E.coli.
15-hydroxyprostaglandin dehydrogenase [NAD(+)] (EC:1.1.1.141), 15-PGDH, Hpgd, Pgdh1, Prostaglandin dehydrogenase 1.
Greater than 95.0% as determined by SDS-PAGE.
Description
HPGD Mouse Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (1-269) and having a molecular mass of 31.6kDa.
HPGD is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
15-hydroxyprostaglandin dehydrogenase [NAD(+)] (EC:1.1.1.141), 15-PGDH, Hpgd, Pgdh1, Prostaglandin dehydrogenase 1.
E.coli.
MGSSHHHHHH SSGLVPRGSH MGSMHVNGKV ALVTGAAQGI GKAFAEALLL HGAKVALVDW NLEAGVKCKA ALDEQFEPQK TLFVQCDVAD QKQLRDTFRK VVDHFGRLDI LVNNAGVNNE KNWEQTLQIN LVSVISGTYL GLDYMSKQNG GEGGIIINMS SLAGLMPVAQ QPVYCASKHG IIGFTRSAAM AANLMKSGVR LNVICPGFVD TPILESIEKE ENMGQYIEYK DQIKAMMKFY GVLHPSTIAN GLINLIEDDA LNGAIMKITA SKGIHFQDYD ISPLLVKAPL TS.
Product Science Overview
15-PGDH is responsible for the oxidation of the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in the loss of biological activity of these molecules . Prostaglandins are lipid compounds that have diverse hormone-like effects in animals. They are involved in various physiological processes such as inflammation, blood flow, the formation of blood clots, and the induction of labor.
The enzyme uses NAD+ (nicotinamide adenine dinucleotide) as a cofactor for its dehydrogenase activity. The reaction catalyzed by 15-PGDH is crucial for the catabolism of prostaglandins, thereby regulating their levels and activity within the body .
In mice, the HPGD gene is located on chromosome 8 . The expression of 15-PGDH is observed in various tissues, including the right lung lobe, seminal vesicle, wall of the urinary bladder, and the mucosa of the urinary bladder . This widespread expression indicates the enzyme’s significant role in multiple physiological processes.
15-PGDH has been shown to have essential roles in the prevention of early termination of pregnancy and maternal morbidity in mice . Knockout studies in mice have demonstrated that the absence of 15-PGDH leads to early pregnancy termination and maternal morbidity, highlighting its importance in reproductive health.
The recombinant form of 15-PGDH (Mouse) is produced using recombinant DNA technology, which involves inserting the HPGD gene into a suitable expression system to produce the protein in large quantities. This recombinant protein is used in various research applications to study the enzyme’s function, regulation, and potential therapeutic uses.
Mutations in the HPGD gene have been associated with several human diseases, including hypertrophic osteoarthropathy, primary autosomal recessive, and patent ductus arteriosus . The study of the mouse recombinant form of 15-PGDH provides valuable insights into the enzyme’s role in these conditions and its potential as a therapeutic target.
