HPD Mouse
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, F Alloantigen, F protein.
Description
HPD Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 416 amino acids (1-393 a.a) and having a molecular mass of 47.4kDa.
HPD is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, F Alloantigen, F protein.
MGSSHHHHHH SSGLVPRGSH MGSMTTYNNK GPKPERGRFL HFHSVTFWVG NAKQAASFYC NKMGFEPLAY RGLETGSREV VSHVIKQGKI VFVLCSALNP WNKEMGDHLV KHGDGVKDIA FEVEDCDHIV QKARERGAKI VREPWVEQDK FGKVKFAVLQ TYGDTTHTLV EKINYTGRFL PGFEAPTYKD TLLPKLPRCN LEIIDHIVGN QPDQEMQSAS EWYLKNLQFH RFWSVDDTQV HTEYSSLRSI VVTNYEESIK MPINEPAPGR KKSQIQEYVD YNGGAGVQHI ALKTEDIITA IRHLRERGTE FLAAPSSYYK LLRENLKSAK IQVKESMDVL EELHILVDYD EKGYLLQIFT KPMQDRPTLF LEVIQRHNHQ GFGAGNFNSL FKAFEEEQAL RGNLTDLEPN GVRSGM.
Product Science Overview
4HPPD is a non-heme iron-containing enzyme that typically forms homodimers in eukaryotes, including mammals . The enzyme’s active site contains an iron ion, which is essential for its catalytic activity. The reaction mechanism involves the oxidative decarboxylation of 4-hydroxyphenylpyruvate, leading to the formation of homogentisate .
In mammals, 4HPPD is involved in the catabolism of tyrosine, an aromatic amino acid. The enzyme’s activity is crucial for preventing the accumulation of toxic intermediates that can result from tyrosine metabolism . Defects in the gene encoding 4HPPD can lead to metabolic disorders such as tyrosinemia type 3 and hawkinsinuria .
Recombinant 4HPPD is used in various research applications, including the study of tyrosine metabolism and the development of herbicides. In plants, 4HPPD is involved in the biosynthesis of prenylquinones, which are essential for photosynthesis . Inhibitors of 4HPPD are used as herbicides to control weed growth by disrupting this pathway .
