HOXA9 Human

Homeobox A9 Human Recombinant
Cat. No.
BT21861
Source
Escherichia Coli.
Synonyms
Homeobox A9, HOX1G, Homeobox Protein Hox-1G, Homeodomain Protein HOXA9, Homeobox Protein Hox-A9, Homeo Box A9, HOX1.7, ABD-B, HOX1, HOXA9.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HOXA9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 295 amino acids (1-272) and having a molecular mass of 32.6 kDa.
HOXA9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The HOXA9 gene belongs to the Abd-B homeobox gene family and encodes the HOXA9 protein, a transcription factor crucial for hematopoiesis and implicated in leukemia development. High HOXA9 expression is a hallmark of acute myeloid leukemia (AML) and may be sufficient to trigger the disease. Overexpression of HOXA9 significantly expands hematopoietic stem cells. Located within the A cluster on chromosome 7, HOXA9 regulates gene expression, morphogenesis, and differentiation. As a sequence-specific transcription factor, it plays a vital role in a developmental regulatory system that assigns positional identities along the anterior-posterior axis. Furthermore, HOXA9 is essential for the induction of E-selectin and VCAM-1 on endothelial cells at inflammation sites.
Description
Recombinant human HOXA9, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 32.6 kDa. It comprises 295 amino acids, including the HOXA9 sequence (amino acids 1-272) and an N-terminal 23 amino acid His-tag. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The HOXA9 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to store the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Homeobox A9, HOX1G, Homeobox Protein Hox-1G, Homeodomain Protein HOXA9, Homeobox Protein Hox-A9, Homeo Box A9, HOX1.7, ABD-B, HOX1, HOXA9.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMATTGAL GNYYVDSFLL GADAADELSV GRYAPGTLGQ PPRQAATLAE HPDFSPCSFQ SKATVFGASW NPVHAAGANA VPAAVYHHHH HHPYVHPQAP VAAAAPDGRY MRSWLEPTPG ALSFAGLPSS RPYGIKPEPL SARRGDCPTL DTHTLSLTDY ACGSPPVDRE KQPSEGAFSE NNAENESGGD KPPIDPNNPA ANWLHARSTR KKRCPYTKHQ TLELEKEFLF NMYLTRDRRY EVARLLNLTE RQVKIWFQNR RMKMKKINKD RAKDE.

Product Science Overview

Discovery and Significance

The homeobox genes were first discovered in the early 1980s through studies on Drosophila melanogaster (fruit fly) mutations that caused body parts to develop in the wrong locations . This discovery highlighted the importance of homeobox genes in determining the body plan and segment identity during embryonic development .

Structure and Function

HOXA9 is one of the genes located in the HOXA cluster on chromosome 7 in humans. It encodes a transcription factor that binds to specific DNA sequences, regulating the expression of target genes involved in cell growth, differentiation, and apoptosis . The human recombinant HOXA9 protein is produced in E. coli and consists of 295 amino acids, including a 23 amino acid His-tag at the N-terminus . It has a molecular mass of approximately 32.6 kDa .

Clinical Relevance

HOXA9 has been implicated in various cancers, including leukemia and gastric cancer . Aberrant expression of HOXA9 is associated with poor prognosis and aggressive tumor behavior . As a result, HOXA9 is being studied as a potential biomarker and therapeutic target in cancer treatment .

Production and Storage

The recombinant HOXA9 protein is produced using proprietary chromatographic techniques to ensure high purity and stability . It is typically formulated in a buffer containing Tris-HCl, urea, and glycerol, and should be stored at -20°C for long-term stability . To prevent degradation, it is recommended to avoid repeated freeze-thaw cycles and to add a carrier protein for extended storage .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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