HNRNPC Human

Heterogeneous Nuclear Ribonucleoprotein C Human Recombinant
Cat. No.
BT20724
Source
Escherichia Coli.
Synonyms
Heterogeneous Nuclear Ribonucleoprotein C (C1/C2), HNRPC, hnRNP C1/C2, hnRNPC, C1, C2, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

HNRNPC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-293a.a.) and having a molecular mass of 34.5kDa (Molecular weight on SDS-PAGE will appear higher).
HNRNPC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
HNRNPC, a member of the heterogeneous nuclear ribonucleoproteins (hnRNPs) family, is involved in pre-mRNA processing and other aspects of mRNA metabolism and transport within the nucleus. While primarily located in the nucleus, HNRNPC can shuttle between the nucleus and cytoplasm. Notably, HNRNPC possesses specific nucleic acid binding properties.
Description
Recombinant HNRNPC protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 313 amino acids (1-293a.a.) with a molecular mass of 34.5 kDa. It's important to note that the molecular weight observed on SDS-PAGE might be slightly higher. The protein features an N-terminal His-tag of 20 amino acids and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile filtered.
Formulation
The HNRNPC protein is supplied in a solution format at a concentration of 0.25 mg/ml. The formulation buffer consists of 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 5 mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For longer storage, freeze the protein solution at -20°C. To ensure optimal protein stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). Minimize repeated freeze-thaw cycles.
Purity
The purity of HNRNPC is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Heterogeneous Nuclear Ribonucleoprotein C (C1/C2), HNRPC, hnRNP C1/C2, hnRNPC, C1, C2, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS AAEMYGSSFD LDYDFQRDYY DRMYSYPARV PPPPPIARAV VPSKRQRVSG NTSRRGKSGF NSKSGQRGSS KSGKLKGDDL QAIKKELTQI KQKVDSLLEN LEKIEKEQSK QAVEMKNDKS EEEQSSSSVK KDETNVKMES EGGADDSAEE GDLLDDDDNE DRGDDQLELI KDDEKEAEEG EDDRDSANGE DDS.

Product Science Overview

Introduction

Heterogeneous Nuclear Ribonucleoprotein C (hnRNP C) is a member of the heterogeneous nuclear ribonucleoproteins (hnRNPs) family, which are RNA-binding proteins that play crucial roles in the processing and metabolism of RNA within the cell nucleus . These proteins are involved in various aspects of nucleic acid metabolism, including alternative splicing, mRNA stabilization, and transcriptional and translational regulation .

Structure and Function

hnRNP C is known to bind pre-mRNA and nucleate the assembly of 40S hnRNP particles . It interacts with poly-U tracts in the 3’-UTR or 5’-UTR of mRNA, modulating the stability and translation levels of bound mRNA molecules . hnRNP C acts as a tetramer and is involved in the assembly of 40S hnRNP particles . It also functions as a ‘molecular ruler’ in the mRNA processing pathway, committing nascent transcripts from the chromatin template to the mRNA export pathway once the nascent transcript becomes longer than 200-300 nucleotides .

Biological Roles

hnRNP C has multiple biological functions, including transcriptional regulation, DNA repair, and RNA processing . It associates with SWI/SNF and NurD family members to form the locus control region (LCR)-associated remodeling complex (LARC), which binds to the β-globin gene promoter to prevent transcriptional silencing . Additionally, hnRNP C is a key regulator of the BRCA1 and BRCA2 genes, and its depletion can impair S-phase progression of the cell .

Clinical Significance

Diseases associated with hnRNP C include Intellectual Developmental Disorder, Autosomal Dominant 74, and Mixed Connective Tissue Disease . The protein’s involvement in various cellular processes makes it a potential target for RNA-based therapies, especially in the context of neurodegenerative diseases and cancer .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HNRNPA1 Human
Heterogeneous Nuclear Ribonucleoprotein A1 Human Recombinant
Cat. No.
BT20576
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.