HNRNPA1 Human
Heterogeneous Nuclear Ribonucleoprotein A1 Human Recombinant
Cat. No.
BT20576
Source
Escherichia Coli.
Synonyms
Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1, HNRNPA1, HNRPA1, HNRPA1L3, hnRNP-A1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HNRNPA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-320 a.a.) and having a molecular mass of 36.6kDa.
HNRNPA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
HNRNPA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), a member of the ubiquitously expressed hnRNP A/B subfamily, plays a crucial role in RNA metabolism. It participates in pre-mRNA packaging, nuclear export of mature mRNA, and potentially splice site regulation. HNRNPA1 is implicated in HCV RNA replication and may be essential for myometrial protein changes during labor. This protein exhibits unique RNA-binding properties due to its two quasi-RRM domains. As a major hnRNP complex component, HNRNPA1 is primarily located in the nucleoplasm. It shuttles between the nucleus and cytoplasm, likely bound to mRNA, facilitated by its M9 domain, which acts as both a nuclear localization and export signal.
Description
Recombinant human HNRNPA1, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 343 amino acids (1-320 a.a.) with a 36.6 kDa molecular weight. A 23-amino acid His-tag is fused to the N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
This solution contains 0.5 mg/ml of HNRNPA1 protein in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 40% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the solution should be kept at 4°C. For longer storage, it's recommended to freeze the solution at -20°C. To ensure stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
The purity of this product exceeds 85.0% as determined by SDS-PAGE analysis.
Synonyms
Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1, HNRNPA1, HNRPA1, HNRPA1L3, hnRNP-A1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSKSESP KEPEQLRKLF IGGLSFETTD ESLRSHFEQW GTLTDCVVMR DPNTKRSRGF GFVTYATVEE VDAAMNARPH KVDGRVVEPK RAVSREDSQR PGAHLTVKKI FVGGIKEDTE EHHLRDYFEQ YGKIEVIEIM TDRGSGKKRG FAFVTFDDHD SVDKIVIQKY HTVNGHNCEV RKALSKQEMA SASSSQRGRS GSGNFGGGRG GGFGGNDNFG RGGNFSGRGG FGGSRGGGGY GGSGDGYNGF GNDGSNFGGG GSYNDFGNYN NQSSNFGPMK GGNFGGRSSG PYGGGGQYFA KPRNQGGYGG SSSSSSYGSG RRF.
Product Science Overview
Introduction
Structure and Function
hnRNP A1 is one of the most abundant core proteins of hnRNP complexes and plays a crucial role in the regulation of alternative splicing. It has two quasi-RRM domains that bind to RNAs in the N-terminal domain, which are pivotal for RNA specificity and binding. Additionally, it contains a glycine-rich arginine-glycine-glycine (RGG) region known as the RGG box, which enables protein and RNA binding .
The protein is involved in several critical functions:
- Packaging of pre-mRNA into hnRNP particles: This is essential for the proper processing and maturation of mRNA.
- Transport of poly(A) mRNA from the nucleus to the cytoplasm: hnRNP A1 is exported from the nucleus, likely bound to mRNA, and is immediately re-imported.
- Modulation of splice site selection: It influences the selection of splice sites during the processing of pre-mRNA .
Post-Translational Modifications
Clinical Significance
Mutations in the HNRNPA1 gene have been linked to several diseases, including:
Recombinant hnRNP A1
Recombinant hnRNP A1 is produced using recombinant DNA technology, which involves inserting the HNRNPA1 gene into an expression system to produce the protein in a laboratory setting. This recombinant protein is used in various research applications to study its function and role in gene expression and disease .
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