HN1L Human

Hematological and Neurological Expressed 1-Like Human Recombinant
Cat. No.
BT19634
Source
Escherichia Coli.
Synonyms
C16orf34, L11, Hematological and neurological expressed 1-like protein, HN1-like protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HN1L Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a.) and having a molecular mass of 22.5kDa.HN1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Hematological and neurological expressed 1-like (HN1L) is a member of the HN1 family. It is believed to be involved in embryo development and is expressed at varying levels in various tissues, including the liver, kidney, prostate, testis, and uterus.
Description
Recombinant human HN1L, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 213 amino acids (1-190 a.a.) with a molecular weight of 22.5 kDa. It includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The HN1L protein solution (1 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), containing 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
C16orf34, L11, Hematological and neurological expressed 1-like protein, HN1-like protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMFQVPDS EGGRAGSRAM KPPGGESSNL FGSPEEATPS SRPNRMASNI FGPTEEPQNI PKRTNPPGGK GSGIFDESTP VQTRQHLNPP GGKTSDIFGS PVTATSRLAH PNKPKDHVFL CEGEEPKSDL KAARSIPAGA EPGEKGSARK AGPAKEQEPM PTVDSHEPRL GPRPRSHNKV LNPPGGKSSI SFY.

Product Science Overview

Introduction

Hematological and Neurological Expressed 1-Like (HN1L), also known as C16orf34, CRAMP_1-like, or KIAA1426, is a protein encoded by the HN1L gene in humans. This protein has garnered attention due to its involvement in various biological processes and its potential as a therapeutic target in certain cancers.

Gene and Protein Structure

The HN1L gene is located on chromosome 16 and encodes a protein that is approximately 22.5 kDa in size. The recombinant form of this protein, often used in research, is typically expressed in E. coli and purified using conventional chromatography techniques. The recombinant protein is fused to a His-tag at the N-terminus to facilitate purification and detection .

Expression and Function

HN1L is expressed in a variety of tissues, including the liver, kidney, prostate, testis, and uterus, at varying levels . It is proposed to play a role in embryo development, although its exact functions in adult tissues are still being elucidated.

Role in Cancer

Recent studies have highlighted the significance of HN1L in cancer biology, particularly in triple-negative breast cancer (TNBC). TNBC is characterized by its aggressive clinical behavior and resistance to conventional chemotherapy. Research has shown that HN1L is upregulated in a significant proportion of breast cancer cases and is negatively correlated with relapse-free survival in TNBC patients .

HN1L has been identified as a novel therapeutic target for TNBC due to its role in regulating cancer stem cell self-renewal. Silencing HN1L in vitro and in vivo has been shown to suppress the breast cancer stem cell population, sensitize tumors to chemotherapy, and reduce metastasis . Mechanistically, HN1L is believed to function as a transcription factor that regulates the expression of STAT3 and related genes, promoting pathways that support cancer stem cell self-renewal .

Research and Applications

The recombinant form of HN1L is widely used in research to study its function and potential therapeutic applications. It is available from various suppliers and is typically used in studies involving protein-protein interactions, gene expression analysis, and cancer research .

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Cat. No.
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Source
E.coli.
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