HK3 Antibody
Description
Product Specs
Product Science Overview
Hexokinase-3 (HK3) is an enzyme encoded by the HK3 gene located on chromosome 5 in humans . It is one of the four hexokinase isoforms (HK1-4) found in mammalian cells . Hexokinases play a crucial role in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate (G6P), which is the first step in most glucose metabolism pathways .
HK3 is particularly interesting due to its tissue-specific expression patterns. It is predominantly expressed in cells of myeloid origin, such as monocytes and granulocytes . Unlike HK1 and HK2, which are ubiquitously expressed, HK3 expression is significantly associated with myeloid cells . This enzyme has a molecular weight of approximately 100 kDa and is composed of two highly similar 50-kDa domains at its N- and C-terminals .
Recent studies have highlighted the non-glycolytic roles of HK3, particularly in the context of myeloid malignancies . For instance, HK3 has been identified as a transcriptional target of PU.1 (SPI-1), which is upregulated during differentiation therapy of acute promyelocytic leukemia (APL) cell lines . Loss of HK3 function in acute myeloid leukemia (AML) cells has been shown to increase apoptosis and DNA damage, indicating its role in cell survival and therapy resistance .
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various detection systems, such as ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry .
Mouse anti-human antibodies offer increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody . They are essential tools in biomedical research for detecting, sorting, or purifying specific targets .
Combining Hexokinase-3 with Mouse Anti-Human antibodies can be particularly useful in research focused on glucose metabolism and myeloid malignancies. The specificity of mouse anti-human antibodies allows for precise detection and analysis of HK3 expression in human cells, aiding in the understanding of its roles in both glycolytic and non-glycolytic processes.
