HK3 Human

Hexokinase-3 is a 100 kDa protein that is predominantly expressed in myeloid cells . Unlike HK1 and HK2, which can bind to mitochondria and protect against cell death, HK3 does not bind to mitochondria . The recombinant form of HK3 is often produced in Escherichia coli and is available with high purity levels (>95%) and low endotoxin levels (<1.000 Eu/µg) .

HK3 expression is regulated by hypoxia, similar to HK2, but other factors that regulate HK2 do not affect HK3 levels . This regulation is partially dependent on hypoxia-inducible factor (HIF) signaling. HK3 plays a cytoprotective role by reducing oxidant-induced cell death, increasing ATP levels, decreasing the production of reactive oxygen species (ROS), and preserving mitochondrial membrane potential .

Overexpression of HK3 has been shown to reduce oxidant-induced cell death and increase ATP levels . It also decreases the production of reactive oxygen species (ROS) and preserves mitochondrial membrane potential. Additionally, HK3 overexpression is associated with higher levels of transcription factors that regulate mitochondrial biogenesis and greater total mitochondrial DNA content .

Recombinant Human Hexokinase-3 is a full-length protein expressed in Escherichia coli. It is used in various research applications, including studies on glucose metabolism and cellular protection . The recombinant protein is typically tagged with a His tag at the C-terminus for easy purification and is validated for use in SDS-PAGE and HPLC .