HIV-2 gp39 antibody
Description
Product Specs
Product Science Overview
Human Immunodeficiency Virus type 2 (HIV-2) is a retrovirus that can lead to acquired immunodeficiency syndrome (AIDS). It was originally isolated from patients in West Africa and is the dominant form of HIV in that region . HIV-2 shares similarities with HIV-1 in terms of genome structure, transmission, and clinical features, but there are significant differences in their amino acid and nucleotide sequences, particularly in their envelope glycoproteins .
HIV-2 gp39 is an envelope glycoprotein specific to HIV-2. Envelope glycoproteins play a crucial role in the viral infection process by facilitating the entry of the virus into host cells. The gp39 protein assists in the binding of the virus to the CD4 receptor on the surface of target cells, which is a critical step in the viral infection cycle .
Mouse antibodies are commonly used in research due to their specificity and ability to be produced in large quantities. The HIV-2 gp39 mouse antibody is a monoclonal antibody, meaning it is derived from a single clone of cells and recognizes a specific epitope on the gp39 protein .
Production and Purification
The HIV-2 gp39 mouse antibody is typically produced by immunizing mice with recombinant HIV-2 gp39 protein. The antibody-producing cells are then harvested and fused with myeloma cells to create hybridomas, which can be cultured to produce large quantities of the antibody. The antibodies are purified using techniques such as ion exchange chromatography to ensure high purity and specificity .
Applications
The HIV-2 gp39 mouse antibody is used in various research applications, including:
- Western Blotting (WB): To detect the presence of HIV-2 gp39 protein in samples .
- Enzyme-Linked Immunosorbent Assay (ELISA): To quantify the amount of HIV-2 gp39 protein in samples .
- Immunohistochemistry (IHC): To visualize the localization of HIV-2 gp39 protein in tissue sections.
Storage and Handling
The antibody is typically shipped in a lyophilized form at ambient temperature and should be stored at 4°C in a dry environment for long-term storage. After reconstitution with water, the antibody should be aliquoted and stored at -20°C if not used within a month .
