HIV-1 gp41 Long, Biotin
Description
The E.coli derived biotin labeled recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 gag p41 immunodominant regions, amino acids 444-833.The HIV-1 p41 Biotinylated is fused to beta-galactosidase (114 kDa) at the N-terminus.
Product Specs
Product Science Overview
Human Immunodeficiency Virus type 1 (HIV-1) is a retrovirus that primarily infects vital cells in the human immune system, such as helper T cells (specifically CD4+ T cells), macrophages, and dendritic cells. The virus leads to a progressive failure of the immune system, making the body more susceptible to opportunistic infections .
HIV-1 gp41 is a transmembrane glycoprotein that plays a crucial role in the virus’s ability to infect host cells. It is part of the envelope protein complex, which also includes gp120. The gp41 protein facilitates the fusion of the viral membrane with the host cell membrane, a critical step in the viral entry process .
The recombinant HIV-1 gp41 Long is a biotin-labeled protein derived from Escherichia coli (E. coli). This recombinant protein is a non-glycosylated polypeptide chain containing the immunodominant regions of HIV-1 gp41, specifically amino acids 444-833 . The biotin label allows for easy detection and purification in various experimental applications.
- Source: Escherichia coli
- Physical Appearance: Sterile filtered, colorless clear solution
- Formulation: 8M Urea, 20mM Tris-HCl pH 8.0, 10mM β-mercaptoethanol
- Purity: Greater than 95.0% as determined by HPLC analysis and SDS-PAGE
- Stability: Stable at 4°C for one week, should be stored below -18°C to prevent freeze-thaw cycles .
The HIV-1 gp41 Long recombinant protein is highly immunoreactive with sera from HIV-1 infected individuals. It is suitable for use in various laboratory techniques, including:
