HGF Human, HEK
Greater than 90.0% as determined by SDS-PAGE.
Description
HGF Human Recombinant produced in HEK cells is a glycosylated disulfide-linked heterodimer, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 80kDa.
The HGF is purified by proprietary chromatographic techniques.
Product Specs
Recombinant human HGF, produced in HEK cells, is a glycosylated heterodimer with a disulfide bond. It comprises 697 amino acids (Gln-32 to Ser-728) and has a molecular weight of 80kDa.
The purification of HGF is achieved through specific chromatographic methods.
The HGF was lyophilized from a solution containing 10mM Sodium phosphate, 150mM NaCl, 0.01% Tween 80, and 100mM L-Arginine at a pH of 6.5. The concentration of HGF in this solution was 1mg/ml.
To reconstitute the lyophilized Hepatocyte Growth Factor, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration not less than 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
The purity of the HGF is determined to be greater than 90.0% using SDS-PAGE analysis.
The biological activity of HGF was assessed through a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells (ATCC CCL-208). The EC50, which represents the concentration of HGF required to achieve 50% of the maximum effect, is typically in the range of 20-40ng/ml.
Product Science Overview
Hepatocyte Growth Factor (HGF) is a multifunctional cytokine that plays a crucial role in regulating cell growth, motility, and morphogenesis. It is particularly significant in liver regeneration and tissue repair. HGF is secreted by mesenchymal cells and acts on various epithelial cells by binding to the c-Met receptor, a proto-oncogenic tyrosine kinase .
Recombinant Human Hepatocyte Growth Factor (rh-HGF) produced in HEK (Human Embryonic Kidney) cells is a heterodimeric glycoprotein consisting of two polypeptide chains: the α-chain and the β-chain, held together by a single disulfide bond. The α-chain comprises 463 amino acid residues and four kringle domains, while the β-chain consists of 234 amino acid residues .
HGF is a potent mitogen for mature parenchymal hepatocytes and has a broad spectrum of activities, including:
- Induction of Cell Proliferation: HGF stimulates the proliferation of hepatocytes and other epithelial cells.
- Cell Motility and Morphogenesis: It promotes cell movement and the formation of structures during tissue development and repair.
- Anti-apoptotic Effects: HGF acts as an anti-apoptotic factor, protecting cells from programmed cell death.
- Liver Regeneration: It is crucial for liver regeneration processes, especially after partial hepatectomy or liver injuries .
Due to its regenerative properties, HGF has been explored as a therapeutic agent for treating various liver diseases, including fulminant hepatitis and late-onset hepatic failure. Clinical trials have investigated the safety, pharmacokinetics, and efficacy of rh-HGF in patients with these conditions. Although some studies have shown promising results, further research is needed to establish its clinical efficacy .
Recombinant HGF produced in HEK cells is typically formulated as a lyophilized (freeze-dried) powder. It is soluble in water and most aqueous buffers, and it is stable when stored desiccated below 0°C. Reconstituted HGF should be stored in working aliquots at –20°C to –70°C to avoid repeated freeze-thaw cycles .
