HGF Mouse

Hepatocyte Growth Factor Mouse Recombinant
Cat. No.
BT16021
Source

Sf9, Baculovirus cells.

Synonyms

hepatocyte growth factor receptor, HGF R/c-MET, Met, AI838057, c-Met, HGF, HGFR, Par4, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HGF Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 1146 amino acids (25-931aa) and having a molecular mass of 127.8kDa. HGF is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a pleiotropic cytokine primarily produced by mesenchymal cells that exerts its effects on epithelial and endothelial cells. This paracrine factor plays a crucial role in cellular growth, motility, and morphogenesis. Beyond its primary targets, HGF is also expressed in hematopoietic progenitor cells and T cells, contributing to their functions. During embryonic development, HGF is essential for organogenesis, particularly in muscle formation (myogenesis). In adult tissues, HGF actively participates in tissue repair processes, including organ regeneration and wound healing.
Description
Recombinant Mouse HGF, expressed in a Baculovirus system, is a single glycosylated polypeptide chain. This protein consists of 1146 amino acids (with a sequence spanning from amino acid 25 to 931) and exhibits a molecular weight of 127.8 kDa. For purification purposes, the HGF protein is engineered with a C-terminal fusion tag comprising a 239 amino acid sequence (hIgG-His-Tag). The recombinant HGF protein undergoes purification using proprietary chromatographic methods to ensure high purity.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The HGF protein solution is provided at a concentration of 0.25 mg/ml in a buffer composed of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the HGF protein should be stored at 4°C. For extended storage periods, it is recommended to store the protein in frozen aliquots at -20°C. To maintain protein stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the HGF protein is determined to be greater than 85.0% as assessed by SDS-PAGE analysis.
Synonyms

hepatocyte growth factor receptor, HGF R/c-MET, Met, AI838057, c-Met, HGF, HGFR, Par4, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met.

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ECKEALVKSE MNVNMKYQLP NFTAETPIQN VVLHGHHIYL GATNYIYVLN DKDLQKVSEF KTGPVLEHPD CLPCRDCSSK ANSSGGVWKD NINMALLVDT YYDDQLISCG SVNRGTCQRH VLPPDNSADI QSEVHCMFSP EEESGQCPDC VVSALGAKVL LSEKDRFINF FVGNTINSSY PPGYSLHSIS VRRLKETQDG FKFLTDQSYI DVLPEFQDSY PIKYIHAFES NHFIYFLTVQ KETLDAQTFH TRIIRFCSVD SGLHSYMEMP LECILTEKRR KRSTREEVFN ILQAAYVSKP GANLAKQIGA SPSDDILFGV FAQSKPDSAE PVNRSAVCAF PIKYVNDFFN KIVNKNNVRC LQHFYGPNHE HCFNRTLLRN SSGCEARSDE YRTEFTTALQ RVDLFMGRLN QVLLTSISTF IKGDLTIANL GTSEGRFMQV VLSRTAHLTP HVNFLLDSHP VSPEVIVEHP SNQNGYTLVV TGKKITKIPL NGLGCGHFQS CSQCLSAPYF IQCGWCHNQC VRFDECPSGT WTQEICLPAV YKVFPTSAPL EGGTVLTICG WDFGFRKNNK FDLRKTKVLL GNESCTLTLS ESTTNTLKCT VGPAMSEHFN VSVIISNSRE TTQYSAFSYV DPVITSISPR YGPQAGGTLL TLTGKYLNSG NSRHISIGGK TCTLKSVSDS ILECYTPAQT TSDEFPVKLK IDLANRETSS FSYREDPVVY EIHPTKSFIS GGSTITGIGK TLNSVSLPKL VIDVHEVGVN YTVACQHRSN SEIICCTTPS LKQLGLQLPL KTKAFFLLDG ILSKHFDLTY VHNPVFEPFE KPVMISIGNE NVVEIKGNNI DPEAVKGEVL KVGNQSCESL HWHSGAVLCT VPSDLLKLNS ELNIEWKQAV SSTVLGKVIV QPDQNFALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.

Product Science Overview

Introduction

Hepatocyte Growth Factor (HGF) is a multifunctional cytokine that plays a crucial role in various biological processes, including cell growth, motility, and morphogenesis. It is secreted by mesenchymal cells and primarily acts on cells of epithelial origin. The recombinant form of HGF, particularly from mouse sources, is widely used in research and therapeutic applications.

Discovery and Historical Background

Hepatocyte Growth Factor was first identified in 1984 as a potent mitogen for rat hepatocytes in primary culture . The primary structure of HGF was elucidated through cDNA cloning in 1989, revealing it as a novel growth factor . Around the same time, a factor known as scatter factor was identified from fibroblast-cultured media, which induced scattering in epithelial cells. Subsequent biochemical analyses revealed that HGF and scatter factor were identical .

Structure and Function

The HGF gene is located on chromosome 7q21.1 and comprises 18 exons and 17 introns . Mature HGF is a heterodimer composed of disulfide-linked alpha and beta chains. The alpha chain contains an N-terminal hairpin domain and four kringle domains, while the beta chain contains a serine proteinase homology domain . HGF is initially synthesized as an inactive single chain and is activated through cleavage by serine proteinases .

HGF exerts its biological effects by binding to the proto-oncogenic c-Met receptor, a transmembrane protein with an intracellular tyrosine kinase domain . This binding activates a signaling cascade that regulates cell growth, motility, and morphogenesis. The HGF-Met signaling pathway is essential for developmental and physiological processes and has been implicated in tumorigenesis .

Recombinant HGF

Recombinant HGF, particularly from mouse sources, is produced using various expression systems, including insect cells . The recombinant protein is used in numerous research applications to study its effects on cell proliferation, motility, and tissue regeneration. It is also being explored for therapeutic applications in clinical trials .

Applications and Therapeutic Potential

HGF has a central role in angiogenesis, tumorigenesis, and tissue regeneration due to its ability to stimulate mitogenesis, cell motility, and matrix invasion . Recombinant HGF has been used therapeutically in clinical trials to explore its potential in treating various conditions, including liver diseases, cardiovascular diseases, and cancer .

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