Hepatitis B Core antibody
Description
Product Specs
Mouse IgG2b.
Product Science Overview
Hepatitis B is a viral infection caused by the hepatitis B virus (HBV), which primarily affects the liver. The virus has several antigens, including the Hepatitis B core antigen (HBcAg), which is a key marker in the diagnosis and study of HBV infections. The development of monoclonal antibodies against HBcAg, particularly from mice, has been instrumental in advancing research and diagnostic techniques.
Monoclonal antibodies are antibodies that are derived from a single clone of cells and are therefore identical in structure. Mouse monoclonal antibodies against HBcAg are produced by immunizing mice with the HBcAg protein. These antibodies are highly specific to the HBcAg and are used in various diagnostic and research applications .
The production of mouse monoclonal antibodies involves several steps:
- Immunization: Mice are immunized with the HBcAg protein to stimulate an immune response.
- Cell Fusion: Spleen cells from the immunized mice, which produce antibodies, are fused with myeloma cells to create hybridoma cells. These hybridoma cells can proliferate indefinitely and produce large quantities of the desired antibody.
- Screening and Selection: The hybridoma cells are screened to identify those producing the desired antibody. Positive clones are selected and expanded.
- Purification: The monoclonal antibodies are purified from the culture medium for use in various applications .
- Diagnostic Testing: These antibodies are used in immunoassays to detect HBcAg in blood samples, aiding in the diagnosis of HBV infections .
- Research: They are used in research to study the structure and function of HBcAg, as well as the immune response to HBV .
- Therapeutic Development: Research involving these antibodies contributes to the development of therapeutic strategies for HBV infection .
