HCV NS5 Genotype-4

Hepatitis C Virus NS5 Genotype-4 Recombinant
Cat. No.
BT16909
Source
Synonyms
Appearance
Purity
HCV NS5 Genotype-4 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HCV NS5 Genotype 4 immunodominant regions, amino acids 2212-2313. The protein is fused with GST tag at N-terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, positive-sense, single-stranded RNA virus belonging to the Flaviviridae family. It exhibits a high replication rate, producing approximately one trillion particles daily in an infected individual. The absence of proofreading mechanisms in the HCV RNA polymerase results in a remarkably high mutation rate, potentially contributing to its ability to evade the host's immune system. HCV is classified into six genotypes (1-6), each with multiple subtypes, with global variations in their prevalence and distribution. Genotype determination holds clinical significance as it influences the potential response to interferon-based therapy and the required treatment duration. Notably, genotypes 1 and 4 demonstrate reduced responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of HCV NS5 Genotype 4, specifically amino acids 2212-2313. It is expressed with an N-terminal GST tag.
Purity
The purity of the HCV NS5 Genotype-4 protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 1.5M urea, 25mM Tris-HCl (pH 8.0), 0.2% Triton X-100, and 50% glycerol.
Stability
For optimal stability, HCV NS5 Genotype-4 should be stored at -18°C or below. While it can remain stable at 4°C for up to one week, it is crucial to avoid repeated freeze-thaw cycles.
Applications
HCV NS5 Genotype-4 antigen is well-suited for utilization in ELISA and Western blot assays. Its exceptional properties make it an excellent antigen for HCV detection, minimizing specificity concerns.
Purification Method
HCV NS5 Genotype-4 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C virus (HCV) is a significant global health concern, affecting millions of people worldwide. It is a bloodborne virus that primarily targets the liver, leading to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. HCV is classified into seven major genotypes, with genotype 4 being predominantly found in the Middle East, North Africa, and sub-Saharan Africa .

Structure and Function of HCV

HCV is an enveloped, single-stranded positive-sense RNA virus. Its genome encodes a single polyprotein, which is processed into structural and nonstructural proteins. The structural proteins include the core protein and envelope glycoproteins E1 and E2. The nonstructural (NS) proteins, which are crucial for viral replication and assembly, include NS2, NS3, NS4A, NS4B, NS5A, and NS5B .

NS5 Protein

The NS5 region of HCV is divided into two distinct proteins: NS5A and NS5B. NS5A is a multifunctional phosphoprotein involved in viral replication, modulation of the host cell environment, and interaction with host immune responses. NS5B is an RNA-dependent RNA polymerase responsible for the replication of the viral RNA genome .

Genotype 4 and Its Significance

Genotype 4 of HCV is less studied compared to genotypes 1, 2, and 3. However, it is of particular interest due to its high prevalence in certain regions and its unique genetic characteristics. Genotype 4 has several subtypes, and its genetic diversity poses challenges for treatment and vaccine development .

Recombinant Strains

Recombinant strains of HCV arise when two different genotypes or subtypes of the virus exchange genetic material. This can occur through a process called recombination, which can lead to the emergence of new viral strains with unique properties. Recombinant strains can complicate diagnosis, treatment, and vaccine development due to their genetic variability .

NS5 Genotype-4 Recombinant

The NS5 genotype-4 recombinant strain of HCV is a specific recombinant form that includes genetic material from the NS5 region of genotype 4. This recombinant strain is of particular interest to researchers due to its potential impact on viral replication, immune response, and treatment outcomes. Understanding the genetic makeup and behavior of recombinant strains is crucial for developing effective therapeutic strategies .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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