HCV NS5

Hepatitis C Virus NS5 Recombinant
Cat. No.
BT15934
Source
Synonyms
Appearance
Purity
HCV-NS5 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HCV NS5a immunodominant regions, amino acids 2061-2302. The protein is fused with GST at N-terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, single-stranded RNA virus with a positive sense genome. It belongs to the family Flaviviridae. HCV replicates at a high rate, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, leading to a high mutation rate, which helps HCV evade the host's immune response. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of these genotypes vary globally. Genotype determination is crucial clinically as it influences the potential response to interferon-based therapy and its required duration. Genotypes 1 and 4 show a lower response to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant HCV NS5 protein is derived from E. coli and encompasses the immunodominant regions of the HCV NS5a protein, specifically amino acids 2061-2302. A GST tag is fused to the N-terminus of the protein.
Purity
The purity of the HCV-NS5 protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer containing 1.5M Urea, 25mM Tris-HCl (pH 8), 50% glycerol, and 0.2% Triton-X.
Stability
HCV NS5 protein remains stable at 4°C for up to 1 week. However, for long-term storage, it is recommended to store the protein below -18°C. Repeated freeze-thaw cycles should be avoided.
Applications
The HCV-NS5 antigen is suitable for use in various immunological assays, including ELISA and Western blots. It serves as an excellent antigen for the detection of HCV with minimal specificity issues.
Purification Method
HCV-NS5 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for causing chronic liver diseases, including cirrhosis and hepatocellular carcinoma. One of the critical components of HCV is the nonstructural protein 5 (NS5), which plays a vital role in the virus’s replication and pathogenesis.

Hepatitis C Virus Overview

HCV is a single-stranded positive-sense RNA virus belonging to the Flaviviridae family. The HCV genome encodes a single polyprotein, which is processed into structural and nonstructural proteins. The nonstructural proteins, including NS5, are essential for viral replication and assembly .

NS5 Protein

The NS5 protein is divided into two distinct regions: NS5A and NS5B. NS5A is a multifunctional phosphoprotein involved in viral replication, modulation of the host cell environment, and interaction with host immune responses . NS5B, on the other hand, is an RNA-dependent RNA polymerase responsible for replicating the viral RNA genome .

Recombinant NS5

Recombinant NS5 proteins are produced using recombinant DNA technology, where the NS5 gene is cloned and expressed in suitable host cells, such as bacteria or yeast. These recombinant proteins are used in various research and diagnostic applications, including the development of vaccines and antiviral therapies .

Applications of Recombinant NS5
  1. Vaccine Development: Recombinant NS5 proteins are used to develop vaccines that elicit an immune response against HCV. These vaccines aim to generate both humoral and cellular immunity, providing protection against HCV infection .
  2. Diagnostic Tools: Recombinant NS5 proteins are used in diagnostic assays to detect HCV antibodies in infected individuals. These assays help in the early diagnosis and management of HCV infections .
  3. Research: Recombinant NS5 proteins are valuable tools in studying the molecular mechanisms of HCV replication and pathogenesis. They help researchers understand the interactions between the virus and host cells, leading to the development of new therapeutic strategies .

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