HCV NS5 Genotype-3a

Hepatitis C Virus NS5 Genotype-3a Recombinant
Cat. No.
BT16680
Source
Synonyms
Appearance
Purity
HCV NS5 Genotype-3a protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The E.coli derived recombinant protein contains the HCV NS5 Genotype 3a immunodominant regions, amino acids 2212-2313. The protein is fused to a GST tag at N-terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a positive-sense, single-stranded RNA genome. It belongs to the Flaviviridae family. HCV replicates rapidly, producing approximately one trillion particles daily in an infected individual. Its RNA polymerase lacks proofreading ability, leading to a high mutation rate, which contributes to its ability to evade the host's immune response. HCV is classified into six genotypes (1-6), with multiple subtypes within each. The prevalence and distribution of these genotypes vary globally. Genotype determination is clinically significant as it influences the response to interferon-based therapy and the duration of treatment. Genotypes 1 and 4 show a lower response rate compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein is derived from E. coli and encompasses the immunodominant regions of the HCV NS5 protein (Genotype 3a), specifically amino acids 2212-2313. It is expressed with an N-terminal GST tag.
Purity
The purity of the HCV NS5 Genotype-3a protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 1.5M urea, 25mM Tris-HCl (pH 8.0), 0.2% Triton X-100, and 50% glycerol.
Stability
For short-term storage, HCV NS5 Genotype-3a remains stable at 4°C for up to 1 week. However, for long-term storage, it is recommended to store the protein below -18°C. Avoid repeated freeze-thaw cycles.
Applications
The HCV NS5 Genotype-3a antigen is suitable for use in various immunological assays, including ELISA and Western blotting. Its high specificity makes it an excellent antigen for detecting HCV.
Purification Method
HCV NS5 Genotype-3a protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C virus (HCV) is a significant global health concern, responsible for chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). The virus has a single-stranded positive-sense RNA genome that encodes a polyprotein, which is processed into structural and nonstructural proteins. Among these, the nonstructural protein 5A (NS5A) plays a crucial role in the virus’s life cycle and pathogenesis .

Structure and Function of NS5A

NS5A is a multifunctional protein involved in various stages of the HCV life cycle, including viral replication and assembly. It is derived from a large polyprotein translated from the HCV genome and undergoes post-translational processing by the NS3 viral protease . NS5A consists of three domains:

  1. Domain I: Contains zinc-binding and RNA-binding motifs essential for HCV replication.
  2. Domain II: Involved in RNA replication.
  3. Domain III: Plays a role in virus assembly .
Genotype-3a and Its Significance

HCV is classified into several genotypes, with genotype 3a being one of the most prevalent worldwide. Genotype-3a is particularly significant due to its association with a higher risk of liver steatosis and a faster progression to cirrhosis compared to other genotypes . The genetic diversity of NS5A in genotype-3a has been implicated in resistance to interferon-based therapies, making it a critical target for antiviral drug development .

Recombinant NS5A Proteins

Recombinant NS5A proteins are produced using various expression systems to study their structure, function, and interactions with host cellular factors. These recombinant proteins are valuable tools for understanding the molecular mechanisms of HCV replication and pathogenesis. They are also used in the development of diagnostic assays and therapeutic interventions .

Research and Therapeutic Implications

Research on NS5A has led to the development of direct-acting antivirals (DAAs) that target this protein, significantly improving the treatment outcomes for HCV-infected patients. However, the emergence of resistance-associated substitutions (RASs) in NS5A poses a challenge to the efficacy of these therapies . Continuous research is essential to identify new inhibitors and develop strategies to overcome resistance.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HCV NS5
Hepatitis C Virus NS5 Recombinant
Cat. No.
BT15934
Source
HCV NS5 Genotype-3
Hepatitis C Virus NS5 Genotype-3 Recombinant
Cat. No.
BT16603
Source
HCV NS5 Genotype-3b
Hepatitis C Virus NS5 Genotype-3b Recombinant
Cat. No.
BT16778
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.