HCV NS5 Genotype 1a

Hepatitis C Virus NS5 Genotype-1a (2322-2423) Recombinant
Cat. No.
BT16050
Source
Synonyms
Appearance
Purity
HCV NS5 Genotype-1a protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HCV NS5 Genotype1a immunodominant regions, amino acids 2322-2423 having a total Mw of 38.34kD which includes a 26Kda GST tag.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50 nm), enveloped, single-stranded RNA virus with a positive sense genome. It belongs to the family Flaviviridae. HCV exhibits a high replication rate, producing approximately one trillion particles daily in an infected individual. The absence of proofreading mechanisms in the HCV RNA polymerase results in a remarkably high mutation rate, contributing to its ability to evade the host's immune response. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of these genotypes vary globally. Genotype determination is clinically crucial as it influences the potential response to interferon-based therapy and the required treatment duration. Notably, genotypes 1 and 4 demonstrate lower responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of the HCV NS5 protein from genotype 1a, specifically amino acids 2322-2423. It has a total molecular weight of 38.34 kDa, including a 26 kDa GST tag.
Purity
The purity of the HCV NS5 Genotype-1a protein exceeds 95%, as determined by 10% PAGE and Coomassie staining.
Formulation
The protein is supplied in a buffer composed of 50mM Tris (pH 8) and 5mM EDTA.
Stability
For optimal stability, HCV NS5 Genotype-1a should be stored below -18°C. While it can remain stable at 4°C for up to one week, it is crucial to avoid repeated freeze-thaw cycles.
Applications
The HCV NS5 Genotype-1a antigen is a versatile reagent suitable for various immunological assays, including ELISA and Western blots. Its exceptional specificity makes it an ideal antigen for the detection of HCV.
Purification Method
HCV NS5 Genotype-1a protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for its ability to cause chronic liver diseases, including cirrhosis and hepatocellular carcinoma. Among the various genotypes of HCV, Genotype-1a is one of the most prevalent and studied. The nonstructural protein 5 (NS5) of HCV plays a crucial role in the virus’s replication and is a target for antiviral therapies.

Hepatitis C Virus Structure

HCV is a single-stranded positive-sense RNA virus that encodes a single polyprotein. This polyprotein is processed into at least 11 polypeptides, including three structural proteins (core, and envelope proteins E1 and E2), a small polypeptide named p7, and six nonstructural (NS) proteins (NS2, NS3, NS4A, NS4B, NS5A, and NS5B) . The NS5 protein is further divided into NS5A and NS5B, both of which are essential for viral replication.

NS5 Protein and Its Importance

The NS5 protein is a multifunctional protein involved in various stages of the HCV life cycle. NS5A, in particular, is known for its role in viral replication and assembly. It is derived from the large polyprotein translated from the HCV genome and undergoes post-translational processing by the NS3 viral protease . NS5A is also involved in modulating the host’s immune response, making it a critical target for antiviral drug development.

Recombinant NS5 Genotype-1a (2322-2423)

The recombinant NS5 protein of HCV Genotype-1a, specifically the region spanning amino acids 2322 to 2423, is of particular interest in research and therapeutic development. This fragment is expressed in Escherichia coli and is used in various applications, including ELISA, Western Blot (WB), and Flow Cytometry . The recombinant protein is highly purified, with a purity greater than 95%, making it suitable for high-throughput screening and other research purposes.

Applications and Research

Recombinant NS5 proteins are used extensively in research to understand the virus’s replication mechanisms and to develop antiviral therapies. Studies have shown that modifications in the NS5A region can significantly impact the virus’s replication efficiency and its interaction with host proteins . Researchers use these recombinant proteins to screen for potential inhibitors that can block the function of NS5A, thereby preventing the virus from replicating.

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