HCV NS5, Biotin
Description
Product Specs
Product Science Overview
Hepatitis C Virus (HCV) is a blood-borne pathogen that causes liver inflammation and can lead to severe liver diseases such as cirrhosis and hepatocellular carcinoma . HCV is primarily transmitted through poor blood and organ donation screening procedures and other poor sanitation practices . The virus has a single-stranded positive-sense RNA genome that encodes a single polyprotein, which is processed into at least 11 polypeptides, including structural and nonstructural proteins .
Among the nonstructural proteins, NS5 is a crucial component of the HCV replication complex. It consists of two subunits: NS5A and NS5B . NS5A is a multifunctional phosphoprotein involved in viral replication, assembly, and modulation of host cell processes . NS5B is an RNA-dependent RNA polymerase responsible for viral RNA synthesis .
Recombinant NS5 proteins are produced using recombinant DNA technology, which involves inserting the gene encoding NS5 into an expression system, such as bacteria or yeast, to produce the protein in large quantities. These recombinant proteins are used in various applications, including research, diagnostics, and vaccine development .
Biotinylation is a process of attaching biotin, a vitamin, to proteins. Biotinylated proteins are useful in various biochemical assays due to the strong affinity between biotin and streptavidin, which allows for easy detection and purification of the biotinylated proteins . Biotinylated NS5 proteins can be used in diagnostic assays to detect antibodies against HCV in patient samples, aiding in the diagnosis of HCV infection .
- Research: Biotinylated NS5 proteins are used in research to study the interactions between NS5 and other viral or host proteins. This helps in understanding the mechanisms of HCV replication and pathogenesis .
- Diagnostics: In diagnostic assays, biotinylated NS5 proteins are used to detect anti-HCV antibodies in patient samples. This is crucial for the early diagnosis and management of HCV infection .
- Vaccine Development: Recombinant NS5 proteins are being explored as potential components of HCV vaccines. They can elicit immune responses that may protect against HCV infection .
