HCV NS3 Genotype-1c

Hepatitis C Virus NS3 Genotype-1c, (1192-1459 a.a.) Recombinant
Cat. No.
BT14202
Source
Synonyms
Appearance
Purity
HCV NS3 Genotype-1c protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant His-Tag fusion protein contains the HCV NS3 immunodominant regions, amino acids 1192-1459.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, single-stranded RNA virus with a positive sense genome. It belongs to the family Flaviviridae. HCV replicates at a high rate, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, resulting in a high mutation rate, which contributes to its ability to evade the host's immune response. HCV is classified into six genotypes (1-6), each with multiple subtypes. The distribution and prevalence of these genotypes vary globally. Genotype determination is clinically significant as it influences the potential response to interferon-based therapy and the treatment duration. Genotypes 1 and 4 show a lower response rate to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein consists of the HCV NS3 immunodominant regions, spanning amino acids 1192 to 1459. It is produced in E. coli and includes a His-Tag for purification.
Purity
The purity of the HCV NS3 Genotype-1c protein exceeds 95%, as assessed by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The protein is supplied in a buffer containing 1.5M urea, 25mM Tris-HCl (pH 8), 0.2% Triton-X, and 50% glycerol.
Stability
For short-term storage (up to one week), HCV NS3 Genotype-1c is stable at 4°C. For long-term storage, it is recommended to store the protein below -18°C. Repeated freeze-thaw cycles should be avoided.
Applications
The HCV NS3 Genotype-1c antigen is suitable for use in ELISA and Western blot assays. It is a highly effective antigen for the detection of HCV with minimal cross-reactivity.
Purification Method
HCV NS3 Genotype-1c protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for its ability to cause chronic liver diseases, including cirrhosis and hepatocellular carcinoma. Among the various proteins encoded by HCV, the non-structural protein 3 (NS3) plays a crucial role in the virus’s life cycle and pathogenesis.

Structure and Function of NS3

The NS3 protein is a multifunctional enzyme with protease, helicase, and NTPase activities. It is essential for the processing of the HCV polyprotein and the replication of the viral RNA. The NS3 protease domain, located in the N-terminal region, cleaves the viral polyprotein at specific sites to generate mature viral proteins. The helicase domain, located in the C-terminal region, unwinds the viral RNA, facilitating its replication.

Genotype-1c and Its Significance

HCV is classified into several genotypes, with genotype 1 being the most prevalent and clinically significant. Genotype 1 is further divided into subtypes, including 1a, 1b, and 1c. Genotype-1c is less common but has been identified in various regions. The NS3 protein of genotype-1c shares similarities with other subtypes but also exhibits unique sequence variations that may influence its enzymatic activities and interactions with host factors.

Recombinant NS3 Protein (1192-1459 a.a.)

The recombinant NS3 protein, encompassing amino acids 1192-1459, represents a specific region of the NS3 protein. This region includes the immunodominant epitopes, making it a valuable tool for diagnostic and research purposes. The recombinant protein is typically expressed in bacterial systems, such as E. coli, and purified using chromatographic techniques .

Applications in Research and Diagnostics

The recombinant NS3 protein is widely used in various applications:

  • Diagnostic Assays: The immunodominant region of NS3 is used in enzyme-linked immunosorbent assays (ELISA) and Western blotting to detect antibodies in HCV-infected individuals .
  • Vaccine Development: The antigenic properties of the NS3 protein make it a potential candidate for vaccine development. Studies have focused on identifying and expressing antigenic sites within the NS3 protein to enhance immune responses .
  • Drug Development: The NS3 protease is a target for direct-acting antiviral (DAA) drugs. Understanding the structure and function of the NS3 protein aids in the design of inhibitors that can effectively block its activity and suppress viral replication .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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