HCV NS3 Genotype-1a

Hepatitis C Virus NS3 Genotype-1a, (1192-1459 a.a.) Recombinant
Cat. No.
BT13961
Source
Synonyms
Appearance
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
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Description

The E.coli derived recombinant protein contains the HCV NS3 immunodominant regions, amino acids 1192-1459. The protein is fused to a GST tag at N-Terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, single-stranded, positive-sense RNA virus classified within the Flaviviridae family. Characterized by a high replication rate, HCV produces approximately one trillion particles daily within an infected individual. The virus exhibits a high mutation rate due to the lack of proofreading mechanisms in its RNA polymerase, enabling it to evade the host's immune responses effectively. HCV is categorized into six genotypes (1-6), each with distinct subtypes. Notably, the prevalence and distribution of these genotypes vary globally. Genotype identification holds clinical significance as it determines the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 demonstrate lower responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of the HCV NS3 protein, specifically amino acids 1192-1459. It is expressed with a GST tag fused to its N-terminus.
Purity
The purity of this protein exceeds 95% as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer composed of 1.5M urea, 25mM Tris-HCl (pH 8.0), 0.2% Triton X-100, and 50% glycerol.
Stability
For optimal storage, HCV NS3 Genotype-1a should be maintained at -18°C. While it remains stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
The HCV NS3 Genotype-1a antigen is suitable for use in various immunological assays, including ELISA and Western blotting. It serves as an excellent antigen for the detection of HCV with minimal specificity issues.
Purification Method
HCV NS3 Genotype-1a protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction to Hepatitis C Virus (HCV)

Hepatitis C Virus (HCV) is a significant global health concern, primarily affecting the liver. It can lead to severe liver diseases such as fibrosis, cirrhosis, hepatocellular carcinoma, and liver failure. HCV was first identified in 1989, and since then, extensive research has been conducted to understand its structure, function, and ways to combat the infection .

Structure and Function of HCV NS3 Protein

The NS3 protein of HCV is a nonstructural protein that plays a crucial role in the virus’s life cycle. It is a multifunctional enzyme with protease, helicase, and nucleoside triphosphatase (NTPase) activities. The NS3 protein is essential for the replication of the viral RNA and the processing of the viral polyprotein .

Genotype-1a and Its Significance

HCV has several genotypes, with genotype 1 being the most prevalent and studied. Within genotype 1, there are subtypes 1a and 1b. Genotype-1a is particularly significant because it is commonly found in North America and Europe and is associated with a higher rate of resistance to antiviral therapies compared to other genotypes .

Recombinant NS3 Genotype-1a (1192-1459 a.a.)

The recombinant NS3 protein of HCV genotype-1a, spanning amino acids 1192 to 1459, is a crucial tool for research and diagnostic purposes. This recombinant protein is typically expressed in Escherichia coli (E. coli) and purified using proprietary chromatographic techniques . The region from amino acids 1192 to 1459 includes the immunodominant regions of the NS3 protein, making it highly immunoreactive with sera from HCV-infected individuals .

Applications and Importance

The recombinant NS3 protein is used in various applications, including:

  • Diagnostic Assays: It is used in enzyme-linked immunosorbent assays (ELISA) and Western blot assays to detect antibodies against HCV in patient sera .
  • Vaccine Development: The immunodominant regions of the NS3 protein are potential targets for vaccine development. Research is ongoing to develop vaccines that can elicit a strong immune response against these regions .
  • Drug Screening: The NS3 protein’s protease and helicase activities make it an important target for antiviral drug development. Inhibitors targeting these activities are being screened and developed to combat HCV infection .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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