HCV Mosaic-B

Hepatitis C Virus Mosaic Antigen-B Recombinant
Cat. No.
BT13223
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HCV Mosaic-B protein contains a long core peptide residues 1-120a.a., 1192-1415a.a. of NS3, three epitopes from NS4 and two epitopes from NS5 and the genotype of all sequences are 1b.
A specific peptide was identified from each region; the four peptides from each region were linked together and expressed in E .coli. The recombinant protein migrates at 65kDa.

Product Specs

Introduction
Hepatitis C is a type of viral hepatitis, a liver disease, caused by the hepatitis C virus (HCV). HCV is primarily transmitted through contact with infected blood, but it can also be spread through sexual contact with an infected person and from mother to baby during childbirth. The HCV core protein, NS3, NS4, and NS5 proteins are essential for detecting antibodies against HCV.
Description
The HCV Mosaic-B protein consists of a long core peptide (residues 1-120 and 1192-1415), three epitopes from NS4, and two epitopes from NS5. All sequences are genotype 1b. Specific peptides from each region were linked and expressed in E. coli, resulting in a recombinant protein with a molecular weight of 65 kDa.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
Solution of HCV Mosaic-B protein in PBS containing 50 mM arginine.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The protein purity is greater than 95% as determined by SDS-PAGE (12%) with Coomassie blue staining.
Applications
Suitable applications include ELISA and gold conjugation.
Source
Escherichia Coli.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of individuals worldwide. The virus is an enveloped, positive-sense single-stranded RNA virus belonging to the Hepacivirus genus within the Flaviviridae family . HCV infection can lead to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma, making it a critical target for vaccine development .

Structure and Composition

The HCV genome encodes a single polyprotein, which is processed into ten mature proteins, including three structural proteins (core, E1, E2) and seven non-structural proteins (p7, NS2, NS3, NS4A, NS4B, NS5A, NS5B) . The structural proteins are essential for the formation of the viral particle, while the non-structural proteins are involved in viral replication and assembly .

Mosaic Antigen-B Recombinant

The Mosaic Antigen-B Recombinant is a synthetic construct designed to enhance the immune response against HCV. It incorporates multiple epitopes from different HCV proteins to create a broad and robust immune response . This approach aims to overcome the high genetic diversity of HCV, which poses a significant challenge for vaccine development .

Function and Mechanism

The primary function of the Mosaic Antigen-B Recombinant is to elicit both humoral and cellular immune responses. By presenting multiple epitopes, it can stimulate a wide range of immune cells, including B cells and T cells . This broad activation is crucial for generating a strong and long-lasting immune response capable of neutralizing diverse HCV strains .

Applications

The Mosaic Antigen-B Recombinant has several potential applications in the field of HCV research and vaccine development. It can be used as a component of DNA vaccines, recombinant protein vaccines, and virus-like particle (VLP) vaccines . Additionally, it holds promise for use in therapeutic vaccines aimed at treating chronic HCV infections .

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HCV Mosaic-A
Hepatitis C Virus Mosaic Antigen-A Recombinant
Cat. No.
BT13130
Source
Escherichia Coli.
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