HCV Mosaic-A

Hepatitis C Virus Mosaic Antigen-A Recombinant
Cat. No.
BT13130
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HCV Mosaic-A protein contains a short core peptide residue 1-50a.a., 1269-1316a.a. of NS3, three epitopes from NS4 and two epitopes from NS5 and the genotype of all sequences are 1b.
A specific peptide was identified from each region; the four peptides from each region were linked together and expressed in E .coli. The recombinant protein migrates at 33kDa.

Product Specs

Introduction
Hepatitis C is a type of viral hepatitis, a liver disease, caused by the hepatitis C virus (HCV). HCV is primarily transmitted through contact with infected blood, but it can also spread through sexual contact with an infected person and from mother to baby during childbirth. The detection of antibodies against HCV relies on the presence of HCV core protein, NS3, NS4, and NS5 proteins.
Description
HCV Mosaic-A protein is a recombinant protein engineered to include specific epitopes from the HCV virus. It comprises a short core peptide (residues 1-50), a fragment of the NS3 protein (residues 1269-1316), three epitopes from NS4, and two epitopes from NS5, all derived from the genotype 1b strain. This protein, with a molecular weight of 33 kDa, is produced in E. coli.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
HCV Mosaic-A protein is supplied in a phosphate-buffered saline (PBS) solution.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the HCV Mosaic-A protein is greater than 95%, as determined by SDS-PAGE analysis (12% gel) with Coomassie blue staining.
Applications
This protein is suitable for various applications, including enzyme-linked immunosorbent assays (ELISA) and gold conjugation.
Source
Escherichia Coli.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of individuals worldwide. It is an enveloped, positive-sense single-stranded RNA virus belonging to the Hepacivirus genus within the Flaviviridae family . The virus’s genome encodes a single polyprotein, which is processed into ten mature proteins, including three structural proteins (core, E1, E2) and seven non-structural proteins (p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B) .

Mosaic Antigen-A Recombinant

The development of vaccines against HCV has been challenging due to the virus’s high genetic diversity and the presence of multiple genotypes and subtypes . One promising approach in vaccine development is the use of recombinant antigens, such as the Hepatitis C Virus Mosaic Antigen-A Recombinant. This recombinant antigen is designed to elicit a robust immune response by incorporating multiple viral proteins and epitopes.

Preparation Methods

The preparation of Hepatitis C Virus Mosaic Antigen-A Recombinant involves several steps:

  1. Gene Cloning: The genes encoding the desired viral proteins and epitopes are cloned into suitable expression vectors.
  2. Expression: These vectors are introduced into host cells, such as Escherichia coli or mammalian cells, to produce the recombinant proteins.
  3. Purification: The expressed proteins are purified using techniques like affinity chromatography to obtain high-purity recombinant antigens.
  4. Characterization: The purified proteins are characterized to ensure they retain the desired antigenic properties and conformational integrity.
Chemical Reactions Analysis

The analysis of chemical reactions involving Hepatitis C Virus Mosaic Antigen-A Recombinant focuses on understanding the interactions between the antigen and the host immune system. Key aspects include:

  • Antigenicity: Assessing the ability of the recombinant antigen to induce an immune response.
  • Epitope Mapping: Identifying the specific regions of the antigen recognized by antibodies.
  • Immune Response: Evaluating the cellular and humoral immune responses elicited by the antigen in preclinical and clinical studies.

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HCV Mosaic-B
Hepatitis C Virus Mosaic Antigen-B Recombinant
Cat. No.
BT13223
Source
Escherichia Coli.
THE BIOTEK
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