HCV Core Genotype-4

Hepatitis C Virus Core Genotype-4 Recombinant
Cat. No.
BT12339
Source
Synonyms
Appearance
Purity
HCV Core Genotype-4 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. The protein is fused to a GST tag at N-Terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a single-stranded, positive-sense RNA genome. It belongs to the family Flaviviridae. HCV replicates rapidly, producing approximately one trillion particles daily in an infected individual. Its RNA polymerase lacks proofreading ability, resulting in a high mutation rate that contributes to its ability to evade the host's immune system. HCV is classified into six genotypes (1-6), each with several subtypes. The distribution and prevalence of these genotypes vary globally. Genotype determination is clinically significant as it influences the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 show lower responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant HCV Core protein, derived from E. coli, encompasses the immunodominant regions of the HCV nucleocapsid, specifically amino acids 2-119. It is expressed with an N-terminal GST tag.
Purity
The purity of the HCV Core Genotype-4 protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 50mM Tris-HCl (pH 8.0), 60mM NaCl, 10mM glutathione, 0.25% sarkosyl, and 50% glycerol.
Stability
For short-term storage, HCV Core Genotype-4 is stable at 4°C for up to one week. For long-term storage, it is recommended to store the protein below -18°C. Repeated freeze-thaw cycles should be avoided.
Applications
HCV Core Genotype-4 antigen is suitable for use in various immunological assays, including ELISA and Western blotting. It serves as an excellent antigen for the detection of HCV with minimal specificity issues.
Purification Method
HCV Core Genotype-4 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C virus (HCV) is a significant global health concern, affecting millions of individuals worldwide. It is a bloodborne virus that primarily targets the liver, leading to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. HCV is classified into several genotypes and subtypes, each with distinct geographic distributions and clinical implications. Among these, Genotype 4 is predominantly found in the Middle East and Central Africa .

Hepatitis C Virus Structure

HCV is an enveloped, single-stranded RNA virus belonging to the Flaviviridae family. The viral genome encodes a single polyprotein, which is processed into structural and non-structural proteins. The structural proteins include the core protein and envelope glycoproteins E1 and E2, while the non-structural proteins include NS2, NS3, NS4A, NS4B, NS5A, and NS5B .

Genotype-4 and Its Significance

Genotype-4 (HCV-G4) is one of the less studied genotypes but is highly prevalent in certain regions. It is associated with a higher rate of chronic infection and poses challenges in treatment due to its genetic variability. The development of direct-acting antivirals (DAAs) has significantly improved the treatment outcomes for HCV-G4, but understanding its genetic makeup remains crucial for effective therapeutic strategies .

Recombinant HCV

Recombinant HCV strains are formed through the recombination of genetic material from different HCV genotypes. These recombinants can arise naturally and may exhibit unique properties compared to their parent strains. Recombination events in HCV are relatively rare but have been documented in various studies. The identification of recombinant strains is essential for accurate genotyping and effective treatment planning .

Core Genotype-4 Recombinant

The core protein of HCV plays a vital role in the virus’s life cycle, including viral assembly and immune modulation. Recombinant strains involving the core region of Genotype-4 have been identified, indicating the presence of mosaic genomes derived from different genotypes. These recombinants can complicate the genotyping process and may impact the virus’s behavior and response to treatment .

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