HCC 1 Human, His

HCC-1, also known as CCL14 (C-C motif chemokine ligand 14), is a small cytokine belonging to the CC chemokine family. It is also referred to as Chemokine CC-1/CC-3, HCC-1/HCC-3, NCC-2, and Small-inducible cytokine A14 . This chemokine is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids .

The recombinant form of HCC-1 (CCL14) is often produced with a His tag at the N-terminus, which facilitates purification and detection. The protein is expressed in various systems, including human 293 cells (HEK293) and Escherichia coli (E. coli) . The recombinant protein typically has a molecular weight of approximately 10.7 kDa, but it may migrate as 14-15 kDa under reducing conditions due to glycosylation .

HCC-1 (CCL14) has weak activity on human monocytes and acts via receptors that also recognize MIP-1 alpha . It induces intracellular calcium changes and enzyme release but does not induce chemotaxis at concentrations of 100-1,000 nM . Additionally, it is inactive on T-lymphocytes and neutrophils . Despite its weak activity, HCC-1 plays a role in promoting monocyte, eosinophil, and T-lymphocyte chemotaxis and mediates allergic airway inflammation and cancer .

Recombinant HCC-1 (CCL14) with a His tag is used in various research applications, including studies on chemokine signaling, immune response, and inflammation . The His tag allows for easy purification and detection, making it a valuable tool for laboratory research.

For long-term storage, the recombinant HCC-1 (CCL14) protein should be stored in a lyophilized state at -20°C or lower . After reconstitution, it is recommended to store the protein at -70°C under sterile conditions to maintain stability . Avoiding repeated freeze-thaw cycles is crucial to preserve the protein’s integrity .