HBsAg Paired Antibody

Hepatitis B virus (HBV) is a small, partially double-stranded DNA virus that belongs to the genus Orthohepadnaviruses of the Hepadnaviridae family. It is a bloodborne virus transmitted through contact with infected blood or bodily fluids. HBV infection can lead to liver diseases ranging from acute or chronic hepatitis to cirrhosis and hepatocellular carcinoma .

The surface antigen of the Hepatitis B virus, known as HBsAg, is a protein that forms part of the virus’s outer envelope. It was initially referred to as the Australia antigen when first identified in the 1960s . HBsAg is crucial for the virus’s ability to infect liver cells and is a key marker used in diagnosing HBV infection.

Mouse anti-Hepatitis B surface antigen antibodies are monoclonal antibodies developed to specifically target and bind to HBsAg. These antibodies are produced by immunizing mice with HBsAg and then harvesting the antibody-producing cells. The cells are fused with myeloma cells to create hybridomas, which can be cultured to produce large quantities of the monoclonal antibody .

Paired antibodies refer to two monoclonal antibodies that are used together in immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay), to detect the presence of HBsAg. One antibody, known as the capture antibody, is coated onto a microtiter plate and binds to HBsAg present in the sample. The second antibody, known as the detection antibody, is typically labeled with an enzyme or fluorescent marker and binds to a different epitope on the HBsAg, allowing for the detection and quantification of the antigen .

Mouse anti-HBsAg paired antibodies are widely used in diagnostic assays to detect HBV infection. These assays are crucial for screening blood donations, diagnosing acute and chronic HBV infections, and monitoring the effectiveness of HBV vaccination programs . Additionally, these antibodies are used in research to study the structure and function of HBsAg and to develop new therapeutic strategies for HBV infection.