HAVCR1 Human, HEK

Hepatitis A Virus Cellular Receptor 1 Human Recombinant, HEK
Cat. No.
BT9080
Source
HEK 293.
Synonyms
Hepatitis A Virus Cellular Receptor 1, T-Cell Immunoglobulin Mucin Family Member 1, T-Cell Immunoglobulin Mucin Receptor 1, T-Cell Membrane Protein 1, Kidney Injury Molecule 1, HAVCR-1, TIMD-1, HAVCR, KIM-1, TIM-1, TIMD1, TIM1, KIM1, TIM, T-Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T Cell Immunoglobin Domain And Mucin Domain Protein 1, HAVCR1.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HAVCR1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ser21-Thr288) containing a total of 283 amino acids, having a calculated molecular mass of 30.5kDa. HAVCR1 is fused to a 2 aa N-terminal linker, a 2 aa C-terminal linker and a 6 aa His tag at C-Terminus.

Product Specs

Introduction
Hepatitis A virus cellular receptor 1 (HAVCR1), also known as TIM-1, is a membrane protein that serves as a receptor for both the hepatitis A virus (HHAV) and the protein TIMD4. Primarily found on the surface of renal proximal tubule epithelial cells, HAVCR1 is a type I transmembrane glycoprotein. It plays a role in regulating immune responses and may be involved in asthma and allergic reactions. Interestingly, certain individuals carry a specific variant of the HAVCR1 gene that includes a segment called MTTVP. This segment appears to offer protection against allergies, particularly in those who have been exposed to HHAV.
Description
This product consists of the recombinant human HAVCR1 protein, produced in HEK cells. It is a single-chain polypeptide, with glycosylation, spanning from amino acid Ser21 to Thr288 (total 283 amino acids). This results in a protein with a molecular weight of 30.5 kDa. For purification and tagging purposes, a 2 amino acid linker is added to both the N-terminus and C-terminus, along with a 6 amino acid His tag at the C-terminus.
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
The HAVCR1 protein solution, at a concentration of 0.5 mg/ml in phosphate buffered saline (PBS) with 5% (w/v) Trehalose, undergoes filtration at 0.4 μm before being lyophilized.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of around 0.5 mg/ml. Allow sufficient time for the pellet to dissolve completely. It's important to note that this HAVCR1 product is not sterile. Before using it in cell cultures, it must be filtered using an appropriate sterile filter.
Stability
The lyophilized protein should be stored at -20°C. To avoid repeated freeze-thaw cycles, it's recommended to aliquot the reconstituted protein. While the reconstituted protein can be stored at 4°C for a short period (up to two weeks), with no observable changes within this timeframe, long-term storage at -20°C is recommended.
Purity
The purity of this product is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Hepatitis A Virus Cellular Receptor 1, T-Cell Immunoglobulin Mucin Family Member 1, T-Cell Immunoglobulin Mucin Receptor 1, T-Cell Membrane Protein 1, Kidney Injury Molecule 1, HAVCR-1, TIMD-1, HAVCR, KIM-1, TIM-1, TIMD1, TIM1, KIM1, TIM, T-Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T Cell Immunoglobin Domain And Mucin Domain Protein 1, HAVCR1.
Source
HEK 293.
Amino Acid Sequence
ASSVKVGGEA GPSVTLPCHY SGAVTSMCWN RGSCSLFTCQ NGIVWTNGTH VTYRKDTRYK LLGDLSRRDV SLTIENTAVS DSGVYCCRVE HRGWFNDMKI TVSLEIVPPK VTTTPIVTTV PTVTTVRTST TVPTTTTVPM TTVPTTTVPT TMSIPTTTTV LTTMTVSTTT SVPTTTSIPT TTSVPVTTTV STFVPPMPLP RQNHEPVATS PSSPQPAETH PTTLQGAIRR EPTSSPLYSY TTDGNDTVTE SSDGLWNNNQ TQLFLEHSLL TANTTKLHHH HHH.

Product Science Overview

Structure and Expression

HAVCR1 is a class 1 integral membrane glycoprotein that contains two extracellular domains: a virus-binding immunoglobulin-like (IgV) domain and a mucin-like domain that extends the IgV from the cell membrane . This receptor is widely expressed in various tissues, with the highest levels found in the kidney and testis . It is particularly highly expressed in kidney proximal tubular cells, especially in cases of kidney injury .

Function and Role in Disease

HAVCR1 serves as a cellular receptor for the human hepatitis A virus (HHAV) and is involved in the host’s immune response to viral infections . It also plays a significant role in the regulation of asthma and allergic diseases . In the context of kidney injury, the ectodomain of KIM-1 is cleaved and released into the urine, making it a sensitive and specific biomarker for acute kidney injury (AKI) and chronic kidney disease (CKD) in both rodents and humans .

Recombinant HAVCR1

The recombinant form of HAVCR1, expressed in human embryonic kidney (HEK) 293 cells, is used in various research applications. This recombinant protein is often tagged with histidine (His) for purification purposes and is available in lyophilized form . It is used as an internal standard for bioanalysis of HAVCR1 in mass spectrometry .

Applications in Research

Recombinant HAVCR1 is utilized in studies related to viral infections, immune response regulation, and kidney injury. Its role as a biomarker for kidney injury makes it valuable in both clinical and research settings. Additionally, its involvement in asthma and allergic diseases provides insights into potential therapeutic targets for these conditions .

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