Influenza-A Solomon Islands

H1N1 Influenza-A Virus Solomon Islands/03/06
Cat. No.
BT27398
Source
Synonyms
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Solomon Islands/03/06. The Influenza Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.

Product Specs

Introduction
Influenza A virus subtype H1N1 has mutated into many strains, including those responsible for the Spanish Flu, mild human flus, endemic pig infections, and various avian strains. This virus exists as a roughly 100nm globular particle encased in a lipid bilayer taken from its host's plasma membrane. Embedded within this bilayer are approximately 500 molecules of hemagglutinin ("H") and 100 molecules of neuraminidase ("N"). The virus's interior contains 3000 molecules of matrix protein, 8 RNA segments, numerous nucleoproteins associated with each RNA strand, multiple copies of the three subunits of RNA polymerase, and some "non-structural" proteins with unclear functions.
Description
This product contains allantoic fluid harvested from 10-day-old embryonated eggs that were inoculated with the A/Solomon Islands/03/06 strain of influenza A virus. The virus was then isolated and purified using ultracentrifugation with a 10-40% sucrose gradient.
Inactivation
Inactivated by Thimerosal and beta-propiolactone treatment. This product has undergone inactivation procedures in line with established methods. Standard laboratory practices for safe handling of microbiological and viral agents are mandatory when working with this product.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The H1N1 A/Solomon Islands/03/06 solution is formulated in a buffer composed of 0.1M NaCl, 10mM Tris-HCl, 1mM EDTA (pH 8.0), 0.1% sodium azide (NaN3), and 0.005% thimerosal.
Stability
While the A/Solomon Islands/03/06 virus remains stable at 4°C for up to 4 weeks, it is recommended to store the product at -18°C or lower. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Immunological Activity
Tested with anti-influenza A monoclonal antibodies in ELISA.
Serological studies of influenza A virus, immunogen for antibody production.

Product Science Overview

Introduction

The H1N1 Influenza-A Virus Solomon Islands/03/06 is a strain of the H1N1 subtype of the Influenza A virus. This particular strain was first isolated in the Solomon Islands in 2006. It has been used extensively in research and vaccine development due to its unique properties and relevance in the study of influenza viruses.

Classification and Characteristics
  • Virus Type: Influenza A
  • Subtype: H1N1
  • Strain Name: A/Solomon Islands/3/2006
  • Host: Human
  • Isolation Country: Solomon Islands
  • Collection Date: 2006
  • NCBI Taxon ID: 464623
Genetic and Structural Information

The H1N1 Influenza-A Virus Solomon Islands/03/06 is known for its hemagglutinin (HA) and neuraminidase (NA) proteins, which are critical for the virus’s ability to infect host cells and spread. The HA protein is responsible for binding the virus to the host cell, while the NA protein helps release new viral particles from the host cell.

Epidemiology

This strain was part of the global surveillance and research efforts to monitor and understand the spread and evolution of influenza viruses. The Solomon Islands/03/06 strain has been used as a reference strain in various studies and has contributed to the development of vaccines and antiviral drugs.

Research and Applications

The H1N1 Influenza-A Virus Solomon Islands/03/06 has been utilized in numerous research studies to understand the mechanisms of influenza virus infection, immune response, and vaccine efficacy. It has also been used to produce recombinant proteins for research and diagnostic purposes.

Public Health Impact

The identification and study of this strain have been crucial in the global efforts to combat influenza. By understanding the genetic makeup and behavior of the H1N1 Solomon Islands/03/06 strain, researchers have been able to develop better strategies for prevention, treatment, and control of influenza outbreaks.

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