H1N1 Taiwan

H1N1 Influenza-A Virus Taiwan/1/86
Cat. No.
BT25849
Source
Synonyms
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Taiwan/1/86. The Influenza Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.

Product Specs

Introduction
H1N1, a subtype of the Influenza A virus, encompasses various strains like the Spanish Flu, mild human flu strains, pig strains, and avian strains. This virus, roughly 100nm in diameter, is spherical and enclosed in a lipid bilayer sourced from its host's plasma membrane. This membrane is embedded with approximately 500 hemagglutinin (H) and 100 neuraminidase (N) molecules, which are integral membrane proteins. Internally, the virus contains 3000 matrix protein molecules, 8 RNA segments, and each RNA segment is linked with numerous nucleoprotein copies. Additionally, the virus contains multiple copies of the three subunits of RNA polymerase and some 'non-structural' proteins with undefined functions.
Description
This product contains allantoic fluid extracted from 10-day-old embryonated chicken eggs that were inoculated with the A/Taiwan/1/86 strain of Influenza A virus. The virus was then isolated and purified using ultracentrifugation with a 10-40% sucrose gradient.
Inactivation
This product has been treated with Thimerosal and beta-propiolactone to inactivate the virus. While this treatment is in line with established inactivation methods, standard laboratory practices for safe handling of microbiological and viral agents are still required.
Physical Appearance
This product is a sterile-filtered, colorless solution.
Formulation
The H1N1 A/Taiwan/1/86 solution is formulated with STE buffer, 0.1% sodium azide (NaN3), and 0.005% thimerosal.
Stability
For optimal stability, store A/Taiwan/1/86 below -18°C. While it can remain stable at 4°C for up to 4 weeks, repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates that the purity of this product is greater than 90%.
Immunological Activity
Tested with anti-influenza A monoclonal antibodies in ELISA.
Serological studies of influenza A virus, immunogen for antibody production.

Product Science Overview

Introduction

The H1N1 Influenza-A Virus Taiwan/1/86 is a strain of the H1N1 subtype of the Influenza A virus. This particular strain was first identified in Taiwan in 1986 and has since been studied for its epidemiological and virological characteristics.

Historical Context

The H1N1 subtype of the Influenza A virus has a long history, dating back to the 1918 pandemic, which was one of the deadliest pandemics in human history. The H1N1 virus has undergone significant genetic changes over the years, leading to various outbreaks and pandemics .

Outbreak in 1986

In late October 1986, an outbreak of influenza-like illness was detected at the Naval Air Station in Key West, Florida. The outbreak was associated with airplane travel and affected a squadron that had traveled to Puerto Rico for a temporary assignment. The H1N1 Influenza-A Virus Taiwan/1/86 was recovered from three symptomatic patients during this outbreak . This was the first reported outbreak of respiratory illness due to this strain in the continental United States during the 1986–1987 influenza season.

Virological Characteristics

The H1N1 Influenza-A Virus Taiwan/1/86 is known for its high mutation rates, which is a common characteristic of influenza viruses. These mutations can lead to changes in the virus’s antigenic properties, making it challenging to develop effective vaccines . The strain was considered significantly different from other H1N1 viruses that re-emerged in 1977 .

Epidemiological Impact

The outbreak in 1986 highlighted the potential for rapid spread of the virus through travel and close contact among individuals. Despite the high attack rate among the squadron members, there was no evidence that the outbreak spread to the surrounding civilian communities in Puerto Rico or Key West . This incident underscored the importance of monitoring and controlling influenza outbreaks, especially in military and other close-contact settings.

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