GH Rat
GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH-1.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Description
GH Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21810 Dalton.
GH is purified by proprietary chromatographic techniques.
Product Specs
Growth Hormone (GH) is part of the somatotropin/prolactin hormone family, crucial for growth regulation. This gene, along with four related ones, resides at the gGH locus on chromosome 17, arranged in the same transcriptional orientation. This arrangement likely arose from gene duplications. These five genes share high sequence similarity. Alternative splicing creates additional isoforms of each GH, increasing diversity and specialization potential. Unlike the other four genes at the gGH locus, this specific member is expressed in the pituitary gland but not in placental tissue. Gene mutations or deletions result in GH deficiency and restricted growth.
Recombinant Rat GH, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 190 amino acids, with a molecular weight of 21810 Daltons. The purification of GH is carried out using proprietary chromatographic methods.
For reconstitution of lyophilized Rat GH, sterile 18MΩ-cm H2O is recommended. The initial reconstitution should be at a concentration of not less than 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Lyophilized Rat GH remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, Rat GH should be stored at 4°C for a period of 2-7 days. For future use, it should be stored below -18°C. To ensure stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advisable. Avoid repeated freeze-thaw cycles.
(a) Analysis by Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC).
(b) Analysis by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH-1.
Product Science Overview
Growth hormone (GH) is a pituitary polypeptide hormone synthesized and secreted by somatotroph cells in the anterior pituitary gland. It plays a crucial role in growth, metabolism, and overall development. Recombinant rat growth hormone (rrGH) is a synthetic version of the naturally occurring GH in rats, produced using recombinant DNA technology.
The discovery of GH dates back to the early 20th century, with significant advancements in its synthesis and application occurring over the decades. Recombinant DNA technology, which emerged in the 1970s and 1980s, revolutionized the production of GH. This technology involves inserting the gene responsible for GH production into bacterial or mammalian cells, which then produce the hormone in large quantities.
Recombinant rat growth hormone was developed to study the biological activities and potential therapeutic applications of GH in rats. This synthetic hormone is identical to the naturally occurring GH in rats and is used in various research and clinical settings.
Recombinant rat growth hormone exhibits several biological activities, including:
- Stimulation of Growth: rrGH promotes growth by increasing the size and number of cells in various tissues, particularly bone and muscle.
- Metabolic Effects: It enhances protein synthesis, increases fat metabolism, and influences carbohydrate metabolism.
- Regulation of IGF-1: rrGH stimulates the production of insulin-like growth factor-1 (IGF-1), which mediates many of the growth-promoting effects of GH.
Recombinant rat growth hormone is widely used in research to study the physiological and pathological roles of GH. Some key applications include:
- Growth Studies: Researchers use rrGH to investigate the mechanisms of growth and development in rats.
- Metabolic Research: It is used to study the effects of GH on metabolism, including its role in obesity, diabetes, and other metabolic disorders.
- Endocrine Studies: rrGH helps researchers understand the regulation of GH secretion and its interactions with other hormones.
Studies have shown that rrGH is generally well-tolerated in rats. In long-term studies, high doses of rrGH did not result in significant adverse effects or an increased incidence of tumors . This is consistent with clinical experience in humans, where recombinant human GH (rhGH) has been used safely for decades .
