GH Mahi Mahi
Growth Hormone Mahi Mahi Recombinant
Cat. No.
BT13489
Source
Escherichia Coli.
Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
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Description
Growth Hormone Mahi Mahi Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21810 Dalton.
GH is purified by proprietary chromatographic techniques.
GH is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Growth hormone (GH) is part of the somatotropin/prolactin hormone family, essential for growth regulation. This gene, along with four others, forms the growth hormone locus on chromosome 17. Their shared transcriptional orientation suggests evolutionary gene duplication. These five genes exhibit significant sequence similarity. Alternative splicing further diversifies the five growth hormones, potentially leading to specialized functions. This specific family member is expressed in the pituitary, unlike the other four genes in the growth hormone locus, which are also found in placental tissue. Mutations or deletions within this gene can result in growth hormone deficiency and short stature.
Description
Recombinant Growth Hormone Mahi Mahi, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 190 amino acids, with a molecular weight of 21.81 kDa. Purification of GH is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a 1 mg/ml solution containing 0.0045 mM NaHCO3.
Solubility
Reconstitute the lyophilized Growth Hormone Mahi Mahi with sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized Growth Hormone Mahi Mahi is stable at room temperature for up to 3 weeks. However, it's recommended to store it desiccated below -18°C. After reconstitution, GH Mahi Mahi remains stable at 4°C for 2-7 days. For long-term storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is suggested for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by:
(a) SEC-HPLC analysis.
(b) SDS-PAGE analysis.
Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Source
Escherichia Coli.
Product Science Overview
Structure and Composition
Preparation and Production
The production of Growth Hormone Mahi Mahi Recombinant involves several key steps:
- Gene Cloning: The gene encoding the growth hormone is isolated from Mahi Mahi and inserted into a plasmid vector.
- Transformation: The plasmid is introduced into Escherichia coli cells, which then express the recombinant hormone.
- Fermentation: The transformed E. coli cells are cultured in a controlled environment to produce large quantities of the hormone.
- Purification: The hormone is extracted and purified using chromatographic techniques to achieve a purity greater than 97% .
Physical and Chemical Properties
- Appearance: The hormone is typically provided as a sterile, lyophilized (freeze-dried) white powder .
- Solubility: It is recommended to reconstitute the lyophilized hormone in sterile water to a concentration of at least 100 µg/ml .
- Stability: The lyophilized hormone is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use .
Applications and Usage
Growth Hormone Mahi Mahi Recombinant is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural product, food additive, or household chemical . Researchers utilize this hormone to study growth regulation, metabolic processes, and potential therapeutic applications.
Safety and Handling
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