GM CSF K9
Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant
Cat. No.
BT921
Source
Escherichia Coli.
Synonyms
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by
1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.
1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that plays a crucial role in the production, differentiation, and function of granulocytes and macrophages, which are essential components of the immune system. The active form of GM-CSF exists as a homodimer in the extracellular space. The gene encoding GM-CSF is located within a cluster of related genes on chromosome 5q31. This region is known to be frequently involved in interstitial deletions associated with 5q- syndrome and acute myelogenous leukemia. Notably, this gene cluster also harbors genes responsible for encoding other interleukins, including interleukins 4, 5, and 13. GM-CSF exerts its biological effects by stimulating the growth and differentiation of hematopoietic precursor cells originating from various lineages. These lineages encompass granulocytes, macrophages, eosinophils, and erythrocytes, highlighting its broad impact on hematopoiesis.
Description
Recombinant Canine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 128 amino acids. With a molecular weight of 14.2 kDa, this protein exhibits high purity, achieved through proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile.
Formulation
The protein was lyophilized following extensive dialysis in 1xPBS at a pH of 7.4.
Solubility
To reconstitute the lyophilized GM-CSF, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as required.
Stability
Lyophilized GM-CSF demonstrates stability at room temperature for a period of 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product in a desiccated state at a temperature below -18°C. Once reconstituted, GM-CSF should be stored at 4°C for a period of 2-7 days. For extended storage, it is advisable to add a carrier protein, such as 0.1% HSA or BSA, and store the solution below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 96.0%, as determined by two methods:
1. Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
2. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, determined by measuring the dose-dependent stimulation of human TF1 erythroleukemic cell proliferation, typically falls within the range of 1-4 ng/ml.
Synonyms
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
Source
Escherichia Coli.
Amino Acid Sequence
APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE
GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN
INFKSFKENL KDFLFNIPFD CWKPVKK.
GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN
INFKSFKENL KDFLFNIPFD CWKPVKK.
Product Science Overview
Discovery and Cloning
Function and Mechanism
GM-CSF promotes the survival and activation of macrophages, neutrophils, and eosinophils. It also aids in the maturation of dendritic cells . In vitro studies have shown that canine GM-CSF stimulates the production of granulocyte colony-forming units . When administered in vivo, it causes leukocytosis characterized by mature neutrophilia and, in some cases, monocytosis .
Therapeutic Applications
Research and Development
Recent studies have explored the role of GM-CSF in autoimmune diseases, revealing its pro-inflammatory functions and contribution to the pathogenicity of Th17 cells . This has led to the development of several agents targeting GM-CSF for the treatment of autoimmune diseases, with promising results in clinical trials .
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