GM CSF Monkey

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a cytokine that plays a crucial role in the proliferation, differentiation, and survival of hematopoietic progenitor cells. It is particularly significant in the production and function of granulocytes and macrophages, which are essential components of the immune system. The recombinant form of GM-CSF derived from Rhesus Macaque is used in various research and therapeutic applications due to its similarity to the human form of the cytokine.

GM-CSF is a glycoprotein composed of 127 amino acids. It functions by binding to the GM-CSF receptor, which is expressed on the surface of target cells. This binding triggers a cascade of intracellular signaling pathways that lead to the activation and proliferation of granulocytes and macrophages. These cells are vital for the body’s defense mechanisms, as they are involved in phagocytosis, antigen presentation, and the production of inflammatory mediators.

The recombinant form of GM-CSF from Rhesus Macaque is produced using recombinant DNA technology. The gene encoding GM-CSF is inserted into an expression vector, which is then introduced into a suitable host cell, such as Escherichia coli or Chinese Hamster Ovary (CHO) cells. The host cells are cultured under optimal conditions to produce the recombinant protein, which is then purified using techniques such as affinity chromatography and ion-exchange chromatography.

1. Research: GM-CSF is widely used in immunological research to study the development and function of the immune system. It is also used to investigate the mechanisms of various diseases, including cancer and autoimmune disorders.
2. Therapeutics: Recombinant GM-CSF is used clinically to enhance the recovery of bone marrow function following chemotherapy or bone marrow transplantation. It is also used to treat neutropenia, a condition characterized by low levels of neutrophils, which can lead to increased susceptibility to infections.