GM CSF Human, Sf9
Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, Sf9
Cat. No.
BT794
Source
Insect Cells.
Synonyms
CSF-2, MGI-1GM, GMCSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description
GM-CSF Human Recombinant produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids (18-144) and having a molecular mass of 14.6kDa.
GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.
GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that plays a crucial role in the production, differentiation, and function of granulocytes and macrophages, which are essential components of the immune system. The active form of GM-CSF exists as a homodimer in the extracellular space. The gene encoding GM-CSF is located within a cluster of related genes on chromosome 5q31. This region is known to be susceptible to interstitial deletions associated with 5q- syndrome and acute myelogenous leukemia. Notably, this gene cluster also harbors genes encoding other interleukins, specifically interleukins 4, 5, and 13. GM-CSF exerts its effects by stimulating the growth and differentiation of hematopoietic precursor cells, giving rise to various blood cell lineages, including granulocytes, macrophages, eosinophils, and erythrocytes.
Description
Recombinant Human GM-CSF, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain comprising 127 amino acids (residues 18-144) with a molecular weight of 14.6 kDa. This protein is engineered with a C-terminal His-tag (6x His) to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered.
Formulation
The lyophilized protein is supplied in a phosphate-buffered saline (PBS) matrix.
Solubility
To reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Granulocyte Macrophage Colony Stimulating Factor exhibits stability at room temperature for up to 3 weeks. However, it is advisable to store the lyophilized product desiccated at a temperature below -18°C. Upon reconstitution, GM-CSF should be stored at 4°C for a period of 2-7 days. For long-term storage, it is recommended to aliquot and store the reconstituted protein at -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 98.0% by SDS-PAGE analysis.
Biological Activity
The ED50, determined by the dose-dependent stimulation of human TF-1 cell proliferation (a human erythroleukemic indicator cell line), is less than 0.1 ng/ml. This corresponds to a Specific Activity of 10,000,000 IU/mg.
Synonyms
CSF-2, MGI-1GM, GMCSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
Source
Insect Cells.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
Product Science Overview
Human Recombinant GM-CSF
Human recombinant GM-CSF is a synthetic version of the naturally occurring cytokine. It is produced using recombinant DNA technology, which involves inserting the gene encoding GM-CSF into a host cell to produce the protein in large quantities. The host cells used for this purpose can vary, but one common system is the Sf9 insect cell line.
Production in Sf9 Cells
The Sf9 cell line is derived from the fall armyworm, Spodoptera frugiperda. These cells are commonly used in biotechnology for the production of recombinant proteins. The process involves the following steps:
- Gene Insertion: The gene encoding human GM-CSF is inserted into a baculovirus vector.
- Infection: Sf9 cells are infected with the recombinant baculovirus.
- Protein Expression: The infected Sf9 cells express the human GM-CSF protein.
- Purification: The recombinant GM-CSF is then purified from the cell culture.
Applications and Importance
Recombinant GM-CSF has several important applications:
- Medical Treatments: It is used to boost the immune system in patients undergoing chemotherapy or bone marrow transplants. It helps in the recovery of white blood cells, reducing the risk of infections.
- Research: GM-CSF is used in various research studies to understand its role in immune responses and its potential therapeutic applications.
- Adjuvant Therapy: It has been explored as an adjuvant therapy for invasive fungal diseases, showing promising results in enhancing immune responses .
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