GLP1R Human
Escherichia Coli.
GLP1, GLP2, GRPP
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
GLP1R Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 24-145) containing 132 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 15.55 kDa (calculated).
Product Specs
The glucagon-like peptide-1 receptor (GLP1R) is a protein found on certain brain cells and pancreatic beta cells. When activated, it triggers a cascade that ultimately increases intracellular cAMP levels. In humans, the GLP1R gene, located on chromosome 6, is responsible for its production. GLP1R plays a crucial role in regulating blood sugar by stimulating insulin release. It belongs to the glucagon receptor family, a class of G protein-coupled receptors. Structurally, GLP1R consists of two domains: a transmembrane domain (TMD) that binds to a specific region of GLP-1, and an extracellular domain (ECD) that binds to another region of GLP-1. The TMD houses a specific arrangement of amino acids that influence the receptor's signaling, while the ECD and areas surrounding the TMD contribute to the activation of specific signaling pathways.
This product consists of the GLP1R protein from humans, produced in E. coli bacteria. It is a single chain of 132 amino acids, including a 10 amino acid tag, and has a molecular weight of 15.55 kDa. This protein is not glycosylated.
This product is supplied as a freeze-dried powder. The GLP1R protein was filtered through a 0.4 micrometer filter and freeze-dried from a solution containing 0.5 mg/ml GLP1R in a 50 mM acetate buffer at a pH of 4.0.
To prepare the product for use, it is recommended to first reconstitute the freeze-dried powder in 100 mM acetate buffer to a concentration of approximately 0.5 mg/ml. Any further dilutions should be done using a buffer solution that contains protein.
For long-term storage, the lyophilized protein should be kept at -20°C. After reconstituting the protein, it is recommended to divide it into smaller portions to avoid repeatedly freezing and thawing the product. Once reconstituted, the protein can be stored at 4°C for up to two weeks without degradation.
Purity is greater than 90% as determined by SDS-PAGE analysis.
GLP1, GLP2, GRPP
Escherichia Coli.
MKHHHHHHAS RPQGATVSLW ETVQKWREYR RQCQRSLTED PPPATDLFCN RTFDEYACWP DGEPGSFVNV SCPWYLPWAS SVPQGHVYRF CTAEGLWLQK DNSSLPWRDL SECEESKRGE RSSPEEQLLF LY
Product Science Overview
GLP-1R is composed of two main domains: an extracellular domain (ECD) that binds the C-terminal helix of GLP-1, and a transmembrane domain (TMD) that binds the N-terminal region of GLP-1 . The receptor is primarily found on beta cells of the pancreas and neurons in the brain . It plays a significant role in enhancing insulin secretion in response to elevated blood glucose levels .
The receptor is involved in several biological processes, including:
- Regulation of insulin secretion: GLP-1R activation enhances insulin secretion from pancreatic beta cells .
- Control of blood sugar levels: By promoting insulin release, GLP-1R helps maintain glucose homeostasis .
- Regulation of gastric motility and food intake: GLP-1R influences gastric emptying and appetite, contributing to weight management .
