GIMAP5 Human

GTPase, IMAP Family Member 5 Human Recombinant
Cat. No.
BT12504
Source
Escherichia Coli.
Synonyms
GTPase IMAP family member 5, Immunity-associated nucleotide 4-like 1 protein, Immunity-associated nucleotide 5 protein, IAN-5, hIAN5, Immunity-associated protein 3, GIMAP5, IAN4L1, IAN5, IMAP3, HIMAP3, IAN4, IROD.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GIMAP5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (1-284) and having a molecular mass of 34.4 kDa.
GIMAP5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GTPase, IMAP Family Member 5 (GIMAP5) is a member of the GTP-binding superfamily and the immuno-associated nucleotide subfamily of nucleotide-binding proteins. It plays a role in preventing cell death and is crucial for the maintenance of mitochondrial integrity and the survival of T cells. Variations in the GIMAP5 gene have been linked to systemic lupus erythematosus. Transcriptional read-through can occur between GIMAP5 and the adjacent upstream gene, GIMAP1 (GTPase, IMAP family member 1). GIMAP5 also contributes to the quiescent state of T cells.
Description
Recombinant human GIMAP5, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 307 amino acids (residues 1-284) with a molecular weight of 34.4 kDa. This protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The GIMAP5 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 40% glycerol, and 2 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 85.0% as assessed by SDS-PAGE analysis.
Synonyms
GTPase IMAP family member 5, Immunity-associated nucleotide 4-like 1 protein, Immunity-associated nucleotide 5 protein, IAN-5, hIAN5, Immunity-associated protein 3, GIMAP5, IAN4L1, IAN5, IMAP3, HIMAP3, IAN4, IROD.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGFQRG KYGTMAEGRS EDNLSATPPA LRIILVGKTG CGKSATGNSI LGQPVFESKL RAQSVTRTCQ VKTGTWNGRK VLVVDTPSIF ESQADTQELY KNIGDCYLLS APGPHVLLLV IQLGRFTAQD TVAIRKVKEV FGTGAMRHVV ILFTHKEDLG GQALDDYVAN TDNCSLKDLV RECERRYCAF NNWGSVEEQR QQQAELLAVI ERLGREREGS FHSNDLFLDA QLLQRTGAGA CQEDYRQYQA KVEWQVEKHK QELRENESNW AYKALLRVKH LMLLHYE.

Product Science Overview

Gene and Protein Structure

The GIMAP5 gene is located on chromosome 7q36.1 in humans . The gene encodes a protein that is part of the GIMAP family, which is evolutionarily conserved and involved in various immune functions . The GIMAP family proteins are characterized by their ability to bind GDP/GTP and are predominantly expressed in cells of the hematopoietic system .

Function and Importance

GIMAP5 plays a crucial role in the survival and maintenance of T lymphocytes. In the BioBreeding diabetes-prone (BB-DP) strain of rats, a mutation in the GIMAP5 gene leads to a profound loss of T lymphocytes in secondary lymphoid organs . This discovery highlighted the importance of GIMAP5 in immune function and led to the identification of the GIMAP family of proteins .

Research has shown that GIMAP5 is critical for the survival of peripheral T-cells, hematopoietic stem cells, and progenitor cells . Deficiency in GIMAP5 can lead to a loss of immunological tolerance, which is associated with autoimmune diseases such as systemic lupus erythematosus and type I diabetes .

Mechanisms of Action

The potential mechanisms by which GIMAP5 controls cell survival include the regulation of Bcl-2 family proteins, mitochondrial integrity, lysosomal function, and calcium regulation . These mechanisms are essential for maintaining the long-term survival of T lymphocytes in a quiescent state .

Clinical Relevance

Polymorphisms in the human GIMAP5 gene have been linked to autoimmune diseases, highlighting its clinical significance . Understanding the function and regulation of GIMAP5 can provide insights into the development of therapeutic strategies for autoimmune diseases and other immune-related disorders.

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