GHBP Ovine
Growth Hormone Binding Protein Ovine Recombinant
Cat. No.
BT15048
Source
Escherichia Coli.
Synonyms
GHR, GHBP, GH receptor, Somatotropin receptor.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description
Growth Hormone Binding Protein Ovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 237 amino acids and having a molecular mass of 28 kDa.
GHBP is purified by proprietary chromatographic techniques.
GHBP is purified by proprietary chromatographic techniques.
Product Specs
Introduction
The growth hormone receptor (GHR) is a transmembrane protein that binds to growth hormone (GH) and initiates downstream signaling pathways crucial for growth. A specific variant of this gene, known as GHRd3, lacks exon three and has been extensively studied. Mutations in the GHR gene are linked to Laron syndrome, a growth disorder characterized by short stature, also referred to as growth hormone insensitivity syndrome (GHIS). While other variations exist, including one encoding a soluble form of the receptor (GHRtr), they have not been fully characterized.
Description
Recombinant Ovine Growth Hormone Binding Protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 237 amino acids, resulting in a molecular weight of 28 kDa. The purification process of GHBP involves proprietary chromatographic techniques.
Physical Appearance
White, sterile, lyophilized powder.
Formulation
GHBP is lyophilized from a 1 mg/ml solution containing 0.0045 mM NaHCO3.
Solubility
To reconstitute lyophilized Growth Hormone Binding Protein, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. Further dilutions can be prepared in other aqueous solutions.
Stability
Lyophilized Growth Hormone Binding Protein remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is advised to store it desiccated at a temperature below -18°C. After reconstitution, GHBP should be kept at 4°C for a period of 2-7 days. For future use, it should be stored below -18°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for extended storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of this product is greater than 95%, as determined by SEC-HPLC and SDS-PAGE analysis.
Synonyms
GHR, GHBP, GH receptor, Somatotropin receptor.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.
Product Science Overview
Structure and Function
GHBP is a transmembrane receptor for growth hormone. When GH binds to GHBP, it leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway, which ultimately promotes growth . The binding of GH to GHBP can influence the half-life of GH in circulation and its interaction with target tissues.
Ovine Recombinant GHBP
The ovine recombinant form of GHBP (roGHBP) is produced using recombinant DNA technology. This involves inserting the gene encoding ovine GHBP into a suitable expression system, such as bacteria or yeast, to produce large quantities of the protein. The recombinant protein is then purified for use in research and therapeutic applications.
Applications and Research
Recombinant GHBP has several applications in both research and clinical settings:
- Research Tool: roGHBP is used to study the mechanisms of GH action and its regulation. By understanding how GHBP interacts with GH, researchers can gain insights into the physiological and pathological roles of GH in growth and metabolism.
- Therapeutic Potential: There is potential for using roGHBP in therapeutic applications, such as treating GH deficiencies or conditions where GH activity needs to be modulated. By administering roGHBP, it may be possible to regulate GH levels more precisely.
Industrial Production
The production of recombinant proteins like roGHBP involves several key steps:
- Gene Cloning: The gene encoding ovine GHBP is cloned into an expression vector, which is then introduced into a host organism (e.g., bacteria, yeast).
- Protein Expression: The host organism is cultured under conditions that promote the expression of the recombinant protein.
- Protein Purification: The recombinant protein is extracted and purified using techniques such as affinity chromatography, ensuring high purity and activity.
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