GHBP Human, His

Growth Hormone Binding Protein Human Recombinant, His Tag
Cat. No.
BT14921
Source
Sf9, Insect cells.
Synonyms
Growth Hormone Receptor, Growth Hormone Binding Protein, Somatotropin Receptor GH Receptor, Serum Binding Protein, GHBP, GHIP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GHBP produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 254 amino acids (19-264a.a.) and having a molecular mass of 29.4kDa.
GHBP is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
The growth hormone receptor (GHR) belongs to the cytokine receptor family. Upon binding two GHR molecules, growth hormone triggers signal transduction via receptor dimerization. However, at elevated concentrations, growth hormone exhibits antagonist properties due to the significant disparity in binding affinities at its respective binding sites. This antagonistic effect can be further amplified by diminishing binding at the low-affinity binding site.
Description
Produced in Sf9 insect cells, GHBP is a single, glycosylated polypeptide chain comprising 254 amino acids (19-264a.a.) with a molecular mass of 29.4 kDa. It features an 8-amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The GHBP protein solution is provided at a concentration of 1 mg/ml and contains phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of GHBP is greater than 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The ED50 value, representing the effective concentration for 50% activity, is approximately 10 ng/ml. This is assessed by measuring the ability of GHBP to inhibit GH-induced proliferation in Nb2-11 rat lymphoma cells in the presence of 1.25 ng/ml human growth hormone.
Synonyms
Growth Hormone Receptor, Growth Hormone Binding Protein, Somatotropin Receptor GH Receptor, Serum Binding Protein, GHBP, GHIP.
Source
Sf9, Insect cells.
Amino Acid Sequence
FSGSEATAAI LSRAPWSLQS VNPGLKTNSS KEPKFTKCRS PERETFSCHW TDEVHHGTKN LGPIQLFYTR RNTQEWTQEW KECPDYVSAG ENSCYFNSSF TSIWIPYCIK LTSNGGTVDE KCFSVDEIVQ PDPPIALNWT LLNVSLTGIH ADIQVRWEAP RNADIQKGWM VLEYELQYKE
VNETKWKMMD PILTTSVPVY SLKVDKEYEV RVRSKQRNSG NYGEFSEVLY VTLPQMSQFT CEEDFYLEHH HHHH.

Product Science Overview

Structure and Function

GHBP is a glycoprotein that binds to GH with high affinity, preventing its degradation and prolonging its half-life in the bloodstream. This binding also regulates the amount of free GH available to interact with cell surface receptors, thereby modulating GH signaling pathways. The interaction between GH and GHBP is essential for maintaining proper growth and metabolic functions.

Recombinant GHBP Production

Recombinant GHBP is produced using various expression systems, including bacterial, yeast, insect, and mammalian cells. The human recombinant GHBP with a His tag is commonly expressed in Escherichia coli (E. coli) due to its simplicity and cost-effectiveness. The His tag, a sequence of histidine residues, is added to the protein to facilitate its purification using affinity chromatography techniques.

Expression and Purification

The production of recombinant GHBP involves several steps:

  1. Gene Cloning: The gene encoding the extracellular domain of the human GHR is cloned into an expression vector that includes a sequence for the His tag.
  2. Transformation: The expression vector is introduced into E. coli cells, which then produce the recombinant GHBP.
  3. Induction: The expression of GHBP is induced using specific chemicals, such as IPTG (isopropyl β-D-1-thiogalactopyranoside).
  4. Harvesting: The bacterial cells are harvested and lysed to release the recombinant protein.
  5. Purification: The His-tagged GHBP is purified using nickel affinity chromatography, which selectively binds to the His tag, allowing for the isolation of the recombinant protein.
Applications

Recombinant GHBP is used in various research and clinical applications, including:

  • Studying GH Signaling: GHBP is used to investigate the mechanisms of GH signaling and its regulation.
  • Therapeutic Development: GHBP can be used in the development of therapeutic agents for conditions related to GH deficiency or excess.
  • Diagnostic Tools: GHBP is utilized in diagnostic assays to measure GH levels and assess GH-related disorders.

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