GDF15 D Human

Growth and Differentiation Factor 15 D-Variant Human Recombinant
Cat. No.
BT17815
Source
Escherichia Coli.
Synonyms
GDF-15, MIC1, MIC-1, NAG-1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, Prostate differentiation factor, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, NRG-1, GDF15.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GDF15 D-variant (His substitutes Asp at position 7) Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 2x113 amino acids and having a molecular mass of 24.5kDa.
The GDF15 D-variant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth Differentiation Factor 15 (GDF15), a member of the Transforming Growth Factor Beta (TGF-β) superfamily, plays a crucial role in regulating inflammatory and apoptotic pathways within injured tissues and during disease processes. Primarily produced by the liver, GDF15 expression significantly increases upon damage to organs like the liver, kidneys, heart, and lungs. This protein exhibits diverse functions, promoting cell proliferation and growth arrest, as well as influencing cell differentiation. GDF15 demonstrates neuroprotective effects by activating the Akt pathway and inhibiting the ERK pathway, thereby preventing apoptosis in cerebellar granule neurons. Additionally, GDF15 acts as an autocrine/endocrine factor, counteracting hypertrophy and preserving ventricular performance.
Description
This product consists of recombinant human GDF15, specifically the D-variant where Asparagine at position 7 is replaced with Histidine. Produced in E. coli, this protein is a non-glycosylated homodimer, meaning it comprises two identical polypeptide chains, each containing 113 amino acids. The total molecular weight of the dimer is 24.5 kDa. Purification of the GDF15 D-variant is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, white powder obtained through lyophilization (freeze-drying).
Formulation
The lyophilized GDF15 D-variant is provided without any additional substances or additives.
Solubility
To reconstitute the lyophilized GDF15 D-variant, it is recommended to dissolve it in sterile 5mM acetic acid (AcOH) to a concentration of 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
While the lyophilized GDF15 D-variant remains stable at room temperature for up to 3 weeks, it is advisable to store it desiccated at a temperature below -18°C. Upon reconstitution, the GDF15 solution should be stored at 4°C for a period of 2-7 days. For long-term storage, freezing below -18°C is recommended. To enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin). Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of this product exceeds 95.0%, as determined by two methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
GDF-15, MIC1, MIC-1, NAG-1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, Prostate differentiation factor, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, NRG-1, GDF15.
Source
Escherichia Coli.
Amino Acid Sequence
MARNGDDCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI.

Product Science Overview

Structure and Characteristics

The D-variant of GDF-15 is a non-glycosylated, disulfide-linked homodimer. It consists of two identical 120 amino acid monomers, resulting in a total molecular weight of approximately 26.8 kDa . The protein is produced in Escherichia coli cells and is typically provided as a lyophilized powder for research purposes .

Biological Activity

GDF-15 has been identified as an emerging marker for early heart disease due to its role in regulating inflammatory and apoptotic pathways . The biological activity of the human recombinant GDF-15 D-variant is determined by its ability to inhibit the proliferation of DU-145 cells, with an effective dose (ED50) ranging from 1 to 2 µg/mL .

Applications and Research

Recombinant GDF-15 D-variant is widely used in research to study its effects on various cellular processes, including inflammation, apoptosis, and tissue injury response . It is also being investigated for its potential therapeutic applications in treating heart disease and other conditions associated with cellular stress .

Storage and Stability

The lyophilized form of GDF-15 D-variant should be stored at -20°C in a desiccated state. Once reconstituted, the protein can be stored at 4°C for up to one week or aliquoted and stored at -20°C for long-term use . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .

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