GAGE2D Human
G Antigen 2D Human Recombinant
Cat. No.
BT10009
Source
Escherichia Coli.
Synonyms
G Antigen 2D, Cancer/Testis Antigen 4.8, CT4.8, GAGE-8, GAGE8,
G Antigen 8.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
GAGE2D Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116 a.a) and having a molecular mass of 15.2kDa (Molecular size on SDS-PAGE will appear higher).
GAGE2D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
GAGE2D belongs to the GAGE family, characterized by its organization in clustered repeats and a high degree of predicted sequence identity. However, variations occur through scattered single nucleotide substitutions among family members. The initial GAGE nomenclature was based on identified mRNA sequences. However, distinguishing between products of paralogous genes and polymorphic products became challenging due to the high sequence similarity among GAGE members. Notably, GAGE2D expression is largely absent in normal tissues, with the exception of the testis. Conversely, it exhibits expression in a significant portion of various tumor types.
Description
Recombinant Human GAGE2D, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 139 amino acids. This includes 116 amino acids of the GAGE2D sequence (1-116 a.a) and a 23 amino acid His-tag at the N-terminus. With a molecular weight of 15.2 kDa, the protein might appear larger on SDS-PAGE due to the His-tag. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The GAGE2D protein solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To enhance long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. It's important to minimize repeated freeze-thaw cycles.
Purity
The purity of GAGE2D is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
G Antigen 2D, Cancer/Testis Antigen 4.8, CT4.8, GAGE-8, GAGE8,
G Antigen 8.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT
QRQDPAAAQE GEDEGASAGQ GPKPEADSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC
Product Science Overview
Gene and Protein Structure
The GAGE2D gene encodes a protein that is typically 116 amino acids in length. The recombinant form of this protein is often produced in Escherichia coli (E. coli) and is fused to a His-tag at the N-terminus to facilitate purification . The molecular mass of the recombinant GAGE2D protein is approximately 15.2 kDa .
Expression and Function
GAGE2D is not expressed in normal tissues, except in the testis. However, it is expressed by a large proportion of tumors of various histological origins . This makes GAGE2D a member of the cancer/testis antigen family, which are proteins typically expressed in the testis and various cancers but not in other normal tissues. This selective expression pattern makes GAGE2D a potential target for cancer immunotherapy.
Applications in Research
Recombinant GAGE2D protein is used in various research applications, including:
- Cancer Research: Due to its expression in tumors, GAGE2D is studied for its role in cancer development and as a potential target for cancer immunotherapy.
- Immunology: Researchers investigate the immune response to GAGE2D to develop targeted therapies.
- Protein Interaction Studies: The His-tagged recombinant protein is used in pull-down assays and other techniques to study protein-protein interactions.
Production and Purification
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