GAGE12F Human

GAGE12F genes are organized in clustered repeats and exhibit a high degree of sequence identity. However, they differ by scattered single nucleotide substitutions . The sequences of these genes contain either the antigenic peptide YYWPRPRRY or YRPRPRRY, which are recognized by cytotoxic T-cells .

The recombinant human GAGE12F protein is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117aa) with a molecular mass of 15.4 kDa . The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

The recombinant GAGE12F protein is expressed in E. coli and purified by conventional chromatography techniques . The protein solution typically contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, and 10% glycerol . The purity of the protein is greater than 85% as determined by SDS-PAGE .

GAGE12F’s restricted expression in germ cells and its presence in various tumors make it a valuable marker for cancer research. The antigenic peptides recognized by cytotoxic T-cells suggest its potential role in immune response modulation . This makes GAGE12F a candidate for targeted cancer therapies and diagnostic tools.

For short-term storage, the recombinant GAGE12F protein should be kept at 4°C. For long-term storage, it is recommended to aliquot and store the protein at -20°C to avoid freeze-thaw cycles . Adding a carrier protein such as 0.1% HSA or BSA can enhance stability during long-term storage .