FHL3 Human

Four And A Half LIM Domains 3 Human Recombinant
Cat. No.
BT9510
Source
Escherichia Coli.
Synonyms
Four And A Half LIM Domains 3, SLIM2, Skeletal Muscle LIM-Protein 2, FHL-3, SLIM-2, Four And A Half LIM Domains Protein 3, LIM-Only Protein FHL3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FHL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-280 a.a) and having a molecular mass of 33.6kDa. 
FHL3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Four and a half LIM domains 3 (FHL3) is a protein that belongs to a family characterized by a highly conserved double zinc finger motif known as the four-and-a-half LIM domain. FHL3 has been shown to interact with various proteins, including the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. FHL3 plays a role in tumor suppression, the repression of MyoD expression, and the repression of IgE receptor expression. There are two known transcript variants of this gene, which encode different isoforms.
Description
Recombinant human FHL3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 303 amino acids (with amino acids 1-280 of the FHL3 sequence), has a molecular mass of 33.6 kDa, and is fused to a 23 amino acid His-tag at its N-terminus. It is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
This FHL3 protein solution has a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
The product can be stored at 4°C for 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% via SDS-PAGE analysis.
Synonyms
Four And A Half LIM Domains 3, SLIM2, Skeletal Muscle LIM-Protein 2, FHL-3, SLIM-2, Four And A Half LIM Domains Protein 3, LIM-Only Protein FHL3.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP.

Product Science Overview

Structure and Function

The FHL3 protein contains four complete LIM domains and an additional half LIM domain at the N-terminus. These LIM domains are crucial for protein-protein interactions and play significant roles in various cellular processes . The LIM domains are composed of two tandemly repeated zinc fingers, each binding a zinc atom through conserved cysteine residues .

FHL3 is involved in several biological functions, including:

  • Actin Binding: FHL3 has been shown to bind to actin, a key component of the cytoskeleton .
  • Transcriptional Regulation: FHL3 acts as a transcriptional coactivator, particularly in association with the transcription factor FOXK1 .
  • Tumor Suppression and Oncoprotein Roles: FHL3 can function as both a tumor suppressor and an oncoprotein, depending on the context. It interacts with various cancer-related proteins such as SMAD2, SMAD3, and SMAD4 .
Clinical Significance

FHL3 has been implicated in several diseases, particularly in cancer. Its expression levels can vary significantly in different types of cancers, where it may either suppress tumor growth or promote tumor progression . This dual role makes FHL3 a potential target for cancer therapy, as modulating its activity could influence cancer outcomes .

Research and Applications

Recombinant FHL3 proteins are used in research to study its function and interactions. These studies help in understanding the molecular mechanisms underlying its role in various cellular processes and diseases. The recombinant form of FHL3 is particularly valuable for in vitro experiments, allowing researchers to investigate its properties in a controlled environment.

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FHL2 Human
Four And A Half LIM Domains 2 Human Recombinant
Cat. No.
BT9453
Source
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