FHL2 Human

Four And A Half LIM Domains 2 Human Recombinant
Cat. No.
BT9453
Source
Escherichia Coli.
Synonyms
AAG11, DRAL, FHL-2, SLIM-3, SLIM3, Four and a half LIM domains protein 2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, FHL2, RNA Binding Motif Protein 18, RNA-Binding Motif Protein 18, RBM18.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FHL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-279 a.a) and having a molecular mass of 34.6kDa. FHL2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Four And A Half LIM Domains 2 (FHL2) is a member of the four-and-a-half-LIM-only protein family. These proteins consist of two highly conserved, tandemly arranged zinc finger domains. Each zinc finger contains four highly conserved cysteine residues that bind a zinc atom. FHL2 is believed to be involved in the formation of extracellular membranes. Additionally, FHL2 expression decreases during the transformation of normal myoblasts into rhabdomyosarcoma cells. FHL2 acts as a link between presenilin-2 and an intracellular signaling pathway.
Description
Recombinant human FHL2 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 302 amino acids, including a 23 amino acid His-tag at the N-terminus (1-279 a.a), and has a molecular weight of 34.6 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The FHL2 protein solution is supplied at a concentration of 0.5 mg/ml. It is formulated in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
AAG11, DRAL, FHL-2, SLIM-3, SLIM3, Four and a half LIM domains protein 2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, FHL2, RNA Binding Motif Protein 18, RNA-Binding Motif Protein 18, RBM18.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTERFDC HHCNESLFGK KYILREESPY CVVCFETLFA NTCEECGKPI GCDCKDLSYK DRHWHEACFH CSQCRNSLVD KPFAAKEDQL LCTDCYSNEY SSKCQECKKT IMPGTRKMEY KGSSWHETCF ICHRCQQPIG TKSFIPKDNQ NFCVPCYEKQ HAMQCVQCKK PITTGGVTYR EQPWHKECFV CTACRKQLSG QRFTARDDFA YCLNCFCDLY AKKCAGCTNP ISGLGGTKYI SFEERQWHND CFNCKKCSLS LVGRGFLTER DDILCPDCGK DI.

Product Science Overview

Structure and Function

FHL2 contains two highly conserved, tandemly arranged zinc finger domains, each binding a zinc atom through four conserved cysteines . These LIM domains serve as scaffolds for the assembly of multimeric protein complexes, facilitating interactions with other proteins and playing a crucial role in various cellular processes .

FHL2 is thought to function as a molecular transmitter linking various signaling pathways to transcriptional regulation . It negatively regulates the transcriptional repressor E4F1 and inhibits the transcriptional activity of FOXO1 by enhancing its interaction with SIRT1, leading to FOXO1 deacetylation . Additionally, FHL2 negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes .

Biological Role

FHL2 is involved in the assembly of extracellular membranes and may function as a link between presenilin-2 and intracellular signaling pathways . It plays a role in cell growth, differentiation, and apoptosis, and is implicated in various physiological and pathological processes .

Clinical Significance

Mutations or dysregulation of the FHL2 gene have been associated with several diseases, including Familial Isolated Dilated Cardiomyopathy and Familial Hypertrophic Cardiomyopathy . The protein’s role in transcriptional regulation and signaling pathways makes it a potential target for therapeutic interventions in these conditions.

Research and Applications

Recombinant FHL2 protein is widely used in research to study its function and interactions with other proteins. It is also utilized in assays to investigate its role in various signaling pathways and its potential as a therapeutic target .

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