FGFR1 Human, (22-285)

Fibroblast Growth Factor Receptor-1 Human Recombinant, (22-285 a.a.)
Cat. No.
BT19070
Source

Sf9, Baculovirus cells. 

Synonyms

FGFR-1, bFGF-R, C-FGR, CD331, fms-related tyrosine kinase 2, Pfeiffer syndrome, CEK, FLG, FLT2, KAL2, BFGFR, FGFBR, HBGFR, FGFR1/FGFR1OP2 FUSION GENE, FGFR1/ZNF198 FUSION GENE, FLG FGFR1/BCR FUSION GENE, FLG protein, FMS-LIKE GENE, N-sam tyrosine kinase, basic fibroblast growth factor receptor 1.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FGFR1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 272 amino acids (22-285) and having a molecular mass of 30.4kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). FGFR1 is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Fibroblast Growth Factors (FGFs) are a family of at least 18 structurally similar proteins involved in various physiological and pathological cellular processes. These processes include cell growth, differentiation, angiogenesis, wound healing, and tumorigenesis. FGFs exert their biological activities by binding to and activating a family of type I transmembrane tyrosine kinase receptors (FGFRs). Upon ligand binding, FGFRs dimerize and undergo autophosphorylation, initiating downstream signaling cascades. Alternative splicing of FGFR-1 to -3 mRNAs generates multiple receptor isoforms. A common splicing event results in receptors containing all three immunoglobulin-like domains (IgI, IgII, IgIII), termed the alpha isoform, or only IgII and IgIII, known as the beta isoform. FGFR-3 and FGFR-4 only exhibit the alpha isoform. Further splicing events in FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive exons, produce receptors with alternative IgIII domains (IIIb and IIIc). An IIIa isoform, a secreted FGF-binding protein containing only the N-terminal half of the IgIII domain and some intron sequences, has been reported for FGFR-1. Mutations in FGFR-1 to -3 have been identified in patients with birth defects characterized by craniosynostosis, a condition affecting skull development.
Description
FGFR1 Human Recombinant, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 272 amino acids (22-285) with a molecular weight of 30.4 kDa. The protein appears at approximately 40-57 kDa on SDS-PAGE due to glycosylation. It is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution
Formulation
The FGFR1 solution is provided at a concentration of 0.25 mg/ml in phosphate-buffered saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), store the entire vial at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of FGFR1 Human Recombinant is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms

FGFR-1, bFGF-R, C-FGR, CD331, fms-related tyrosine kinase 2, Pfeiffer syndrome, CEK, FLG, FLT2, KAL2, BFGFR, FGFBR, HBGFR, FGFR1/FGFR1OP2 FUSION GENE, FGFR1/ZNF198 FUSION GENE, FLG FGFR1/BCR FUSION GENE, FLG protein, FMS-LIKE GENE, N-sam tyrosine kinase, basic fibroblast growth factor receptor 1.

Source

Sf9, Baculovirus cells. 

Amino Acid Sequence

RPSPTLPEQD ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG TPNPTLRWLK NGKEFKPDHRIGGYKVRYAT WSIIMDSVVP SDKGNYTCIV ENEYGSINHT YQLDVVERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL EERPAVMTSP LYLELEHHHH HH.

Product Science Overview

Introduction

Fibroblast Growth Factor Receptor-1 (FGFR1) is a crucial protein in the human body, playing a significant role in various biological processes. This article delves into the background of FGFR1, particularly focusing on the human recombinant form spanning amino acids 22-285.

Structure and Function

FGFR1 is a member of the fibroblast growth factor receptor family, which includes FGFR1, FGFR2, FGFR3, and FGFR4 . These receptors are characterized by their highly conserved amino acid sequences and structural domains. FGFR1 consists of an extracellular region with three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain .

The extracellular region of FGFR1 interacts with fibroblast growth factors (FGFs), initiating a cascade of downstream signals that influence cell division, growth, differentiation, and survival . FGFR1 binds both acidic and basic FGFs, playing a pivotal role in limb induction and other developmental processes .

Biological Significance

FGFR1 is involved in several critical biological processes, including:

  • Cell Division and Growth: FGFR1 signaling promotes cell proliferation and growth, essential for tissue development and repair .
  • Embryonic Development: FGFR1 is crucial for proper embryonic development, influencing the formation of various tissues and organs .
  • Wound Healing: FGFR1-mediated signaling aids in wound healing by promoting cell migration and tissue regeneration .
  • Angiogenesis: FGFR1 plays a role in the formation of new blood vessels, a process known as angiogenesis .
Clinical Relevance

Mutations and aberrations in the FGFR1 gene are associated with several disorders and diseases. Some of the notable conditions include:

  • Pfeiffer Syndrome: A genetic disorder characterized by premature fusion of certain skull bones, leading to abnormal head and face shape .
  • Jackson-Weiss Syndrome: A condition involving foot abnormalities and craniosynostosis .
  • Antley-Bixler Syndrome: A disorder affecting skeletal development .
  • Osteoglophonic Dysplasia: A rare skeletal disorder .
  • Kallmann Syndrome: A condition characterized by delayed or absent puberty and an impaired sense of smell .

Chromosomal aberrations involving FGFR1 are also linked to stem cell myeloproliferative disorders and stem cell leukemia lymphoma syndrome .

Recombinant FGFR1 (22-285 a.a.)

The human recombinant form of FGFR1, spanning amino acids 22-285, is a truncated version of the full-length protein. This recombinant form retains the essential extracellular domains required for FGF binding and subsequent signal transduction. It is commonly used in research to study FGFR1’s structure, function, and interactions with ligands and other proteins.

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