FGFR1 Human, His

Fibroblast Growth Factor Receptor-1 Human Recombinant, His Tag
Cat. No.
BT19265
Source

Sf9, Baculovirus cells. 

Synonyms

FGFR-1, bFGF-R, C-FGR, CD331, fms-related tyrosine kinase 2, Pfeiffer syndrome, CEK, FLG, FLT2, KAL2, BFGFR, FGFBR, HBGFR, FGFR1/FGFR1OP2 FUSION GENE, FGFR1/ZNF198 FUSION GENE, FLG FGFR1/BCR FUSION GENE, FLG protein, FMS-LIKE GENE, N-sam tyrosine kinase, basic fibroblast growth factor receptor 1.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FGFR Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 363 amino acids (22-376) and having a molecular mass of 40.4kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). FGFR is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The Fibroblast Growth Factor (FGF) family consists of at least 18 structurally related proteins with diverse roles in physiological and pathological processes, such as cell growth, differentiation, angiogenesis, wound healing, and tumorigenesis. These proteins exert their biological effects by binding to and activating a family of type I transmembrane tyrosine kinase receptors known as FGFRs (FGFR-1 to -4). Upon ligand binding, these receptors dimerize and undergo autophosphorylation, initiating downstream signaling cascades. Alternative splicing of FGFR-1 to -3 mRNAs results in multiple receptor isoforms. Notably, both FGFR-1 and -2 exist as alpha and beta isoforms; the former includes all three immunoglobulin-like (Ig) domains, while the latter contains only IgII and IgIII. In contrast, FGFR-3 and FGFR-4 are found solely as alpha isoforms. Further splicing events in FGFR-1 to -3, specifically in the C-terminal region of the IgIII domain encoded by two mutually exclusive exons, generate receptors with alternative IgIII domains (IIIb and IIIc). A secreted FGF-binding protein, designated as the IIIa isoform, has been identified for FGFR-1. This isoform comprises the N-terminal half of the IgIII domain and some intron sequences. Mutations in FGFR-1, -2, and -3 have been linked to craniosynostosis, a birth defect characterized by premature skull fusion.
Description
Recombinant human FGFR, expressed in Sf9 Baculovirus cells, is a single glycosylated polypeptide chain with a molecular weight of 40.4 kDa (appears as approximately 40-57 kDa on SDS-PAGE). This protein encompasses amino acids 22-376 and features an 8-amino acid His-tag fused to its C-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterile filtered.
Formulation
The FGFR solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing should be minimized.
Purity
The purity is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms

FGFR-1, bFGF-R, C-FGR, CD331, fms-related tyrosine kinase 2, Pfeiffer syndrome, CEK, FLG, FLT2, KAL2, BFGFR, FGFBR, HBGFR, FGFR1/FGFR1OP2 FUSION GENE, FGFR1/ZNF198 FUSION GENE, FLG FGFR1/BCR FUSION GENE, FLG protein, FMS-LIKE GENE, N-sam tyrosine kinase, basic fibroblast growth factor receptor 1.

Source

Sf9, Baculovirus cells. 

Amino Acid Sequence

RPSPTLPEQA QPWGAPVEVE SFLVHPGDLL QLRCRLRDDV QSINWLRDGV QLAESNRTRI TGEEVEVQDS VPADSGLYAC VTSSPSGSDT TYFSVNVSDA LPSSEDDDDD DDSSSEEKET DNTKPNRMPV APYWTSPEKM EKKLHAVPAA KTVKFKCPSS GTPNPTLRWL KNGKEFKPDH RIGGYKVRYA TWSIIMDSVV PSDKGNYTCI VENEYGSINH TYQLDVVERS PHRPILQAGL PANKTVALGS NVEFMCKVYS DPQPHIQWLK HIEVNGSKIG PDNLPYVQIL KTAGVNTTDK EMEVLHLRNV SFEDAGEYTC LAGNSIGLSH HSAWLTVLEA LEERPAVMTS PLYLELEHHH HHH.

Product Science Overview

Introduction

Fibroblast Growth Factor Receptor-1 (FGFR1) is a member of the fibroblast growth factor receptor family, which consists of four membrane-spanning tyrosine kinases (FGFR1-4). These receptors serve as high-affinity receptors for fibroblast growth factors (FGFs), which are involved in various biological processes, including cell growth, differentiation, and tissue repair .

Structure and Function

FGFR1 is composed of an extracellular region with three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain . The extracellular portion of FGFR1 interacts with FGFs, initiating a cascade of downstream signals that influence mitogenesis and differentiation . FGFR1 binds both acidic and basic fibroblast growth factors and plays a crucial role in limb induction .

Recombinant FGFR1 with His Tag

The recombinant form of FGFR1, tagged with a polyhistidine (His) sequence, is produced using various expression systems, such as baculovirus . The His tag facilitates the purification of the protein using affinity chromatography, making it easier to isolate and study the receptor . The recombinant FGFR1 is often used in research to understand its role in cellular processes and its involvement in various diseases .

Biological Significance

FGFR1 is involved in multiple biological processes, including mesoderm induction, cell growth, migration, organ formation, and bone growth . It is alternatively spliced, generating multiple splice variants that are differentially expressed during embryonic development and in the adult body . Mutations in FGFR1 are associated with several diseases, such as Pfeiffer syndrome, idiopathic hypogonadotropic hypogonadism, Kallmann syndrome type 2, osteoglophonic dysplasia, non-syndromic trigonocephaly, Jackson-Weiss syndrome, and Antley-Bixler syndrome .

Research Applications

Recombinant FGFR1 with His tag is widely used in research to study its structure, function, and role in various diseases. It is also used in drug discovery and development, as targeting FGFR1 can have therapeutic potential for conditions related to abnormal FGFR1 signaling .

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